| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 751 | Methanosphaera | TTGCAATAGGACTTGGCGAAA | TGCTCGGAAGATGTGTGGAT | 58.49 | 59.10 | 200 | 81 |
100.00%
|
33.33%
|
| Primer 752 | Methanosphaera | TTGCAATAGGACTTGGCGAAA | ATGCTCGGAAGATGTGTGGA | 58.49 | 59.10 | 201 | 81 |
100.00%
|
33.33%
|
| Primer 753 | Methanosphaera | ACCGGAGCACGTGAAGTAG | GGCCAGAGATCTGCAGCTT | 59.41 | 59.78 | 296 | 81 |
100.00%
|
100.00%
|
| Primer 754 | Methanosphaera | CTGCAGGAGTCGGTGACAT | GGCCAGAGATCTGCAGCTT | 59.41 | 59.78 | 229 | 81 |
100.00%
|
33.33%
|
| Primer 755 | Methanosphaera | ACCGGAGCACGTGAAGTA | GGCCAGAGATCTGCAGCTT | 58.23 | 59.78 | 296 | 81 |
100.00%
|
100.00%
|
| Primer 756 | Methanosphaera | TTAGGGGTAGGACCAGGAGA | TCTGCAGCATCATCCCATGA | 58.30 | 59.16 | 233 | 81 |
100.00%
|
66.67%
|
| Primer 757 | Methanosphaera | CCATCAGAGGACACCTACACA | ACAGCACCTATCACATCCCC | 58.82 | 59.16 | 209 | 81 |
100.00%
|
66.67%
|
| Primer 758 | Methanosphaera | CCATCAGAGGACACCTACACA | AACAGCACCTATCACATCCCC | 58.82 | 59.79 | 210 | 81 |
100.00%
|
66.67%
|
| Primer 759 | Methanosphaera | AGGCACTTGGGGAAATGGA | TTGCTGCCCTTCGTGCTT | 58.83 | 60.20 | 159 | 81 |
100.00%
|
66.67%
|
| Primer 760 | Methanosphaera | TGGATACGGCTGGAGCACA | TGACGGACAGTTCCATGCC | 60.99 | 60.00 | 208 | 81 |
100.00%
|
66.67%
|