| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 781 | Methanosphaera | GCCGTTGCTGCATTACTTGT | CTCCACCTATTGCACCAGC | 59.76 | 58.21 | 163 | 81 |
100.00%
|
66.67%
|
| Primer 782 | Methanosphaera | GCCGTTGCTGCATTACTTGT | AACTCCACCTATTGCACCAGC | 59.76 | 60.61 | 165 | 81 |
100.00%
|
66.67%
|
| Primer 783 | Methanosphaera | TGGCACACTTCAGAGCAGA | GCGGTATGTTGATGCGTGTC | 58.86 | 59.97 | 215 | 81 |
100.00%
|
100.00%
|
| Primer 784 | Methanosphaera | TGGCACACTTCAGAGCAGA | TGCGGTATGTTGATGCGTG | 58.86 | 58.91 | 216 | 81 |
100.00%
|
66.67%
|
| Primer 785 | Methanosphaera | GCAGCACACCCGTATTGT | TCACTCGCTCCAATCTCAGG | 58.02 | 59.18 | 167 | 81 |
100.00%
|
66.67%
|
| Primer 786 | Methanosphaera | TCACCCCAAGATGTAACAGCA | ACTCCCTCATCAAGCTCTTCG | 59.30 | 59.52 | 290 | 80 |
100.00%
|
33.33%
|
| Primer 787 | Methanosphaera | TGCAGCACTAGGAAAACGTGA | TTGCTGGATCTCCTTCGCG | 60.20 | 60.15 | 275 | 80 |
100.00%
|
33.33%
|
| Primer 788 | Methanosphaera | TGCAGCACTAGGAAAACGTG | TTGCTGGATCTCCTTCGCG | 58.77 | 60.15 | 275 | 80 |
100.00%
|
33.33%
|
| Primer 789 | Methanosphaera | TGCAGCTACCAATAGAGGCG | CCTTGCTAGTTCGGGTCCTG | 59.90 | 60.11 | 196 | 80 |
100.00%
|
66.67%
|
| Primer 790 | Methanosphaera | ACTCGGTCCTCAACTTGCA | ATGGTCCCCAAGCAACCA | 58.87 | 58.73 | 182 | 79 |
100.00%
|
33.33%
|