| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 861 | Methanosphaera | CCAACTGAACCTGATAGGGCA | CGAAGTTTGCAGGCATTCCT | 59.72 | 59.12 | 211 | 78 |
100.00%
|
66.67%
|
| Primer 862 | Methanosphaera | TCCAACTGAACCTGATAGGGC | CGAAGTTTGCAGGCATTCCT | 59.44 | 59.12 | 212 | 78 |
100.00%
|
66.67%
|
| Primer 863 | Methanosphaera | GCAAGGGCTATAAGTGCAGTT | CACGACCCTCTTTACTCCACA | 58.63 | 59.38 | 208 | 78 |
100.00%
|
66.67%
|
| Primer 864 | Methanosphaera | GCAAGGGCTATAAGTGCAGTT | ACACGACCCTCTTTACTCCAC | 58.63 | 59.38 | 209 | 78 |
100.00%
|
66.67%
|
| Primer 865 | Methanosphaera | GTGGTAGTGCATGGTGAAACA | TGCCACATGCCCATTACCT | 58.77 | 59.30 | 198 | 78 |
100.00%
|
66.67%
|
| Primer 866 | Methanosphaera | GTTGGGGAAGTTGAACATCCA | AGGATTGCCAGAGACCATGTA | 58.41 | 58.52 | 187 | 77 |
100.00%
|
66.67%
|
| Primer 867 | Methanosphaera | AGTTGGGGAAGTTGAACATCC | AGGATTGCCAGAGACCATGTA | 58.12 | 58.52 | 188 | 77 |
100.00%
|
66.67%
|
| Primer 868 | Methanosphaera | ATCGTTGGAACAGGCGAAGT | TGTGTGTTGTTTGTTGCAGC | 59.97 | 58.29 | 203 | 76 |
100.00%
|
33.33%
|
| Primer 869 | Methanosphaera | TGCTGTGCATTTTCAACCAGG | GCAAGAACCAGGGCTGTCA | 59.93 | 60.23 | 279 | 76 |
100.00%
|
33.33%
|
| Primer 870 | Methanosphaera | ATCCTTCATGGGCTTTTCAGG | GCAAGTTTCAGATTTCCTGGC | 58.26 | 58.05 | 187 | 76 |
100.00%
|
33.33%
|