| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 981 | Methanosphaera | CTGCATGTACAACGCCAGT | ACAAACCCTGCAGCAGCT | 58.46 | 59.80 | 298 | 72 |
100.00%
|
33.33%
|
| Primer 982 | Methanosphaera | GCAGCAATGGAACTACCGGT | AGGGTCTCTATGCCATAGTGG | 60.68 | 58.39 | 216 | 72 |
100.00%
|
33.33%
|
| Primer 983 | Methanosphaera | GGACCACATGCATGTACTGGT | GATGCCCTCAGCTTCCACA | 60.34 | 59.70 | 186 | 72 |
100.00%
|
33.33%
|
| Primer 984 | Methanosphaera | ATTGCAATAGGACTTGGCGAA | TGCTCGGAAGATGTGTGGAT | 58.28 | 59.10 | 201 | 72 |
100.00%
|
33.33%
|
| Primer 985 | Methanosphaera | ATTGCAATAGGACTTGGCGAA | ATGCTCGGAAGATGTGTGGA | 58.28 | 59.10 | 202 | 72 |
100.00%
|
33.33%
|
| Primer 986 | Methanosphaera | ACAAGTGCATACCTATGGGCA | TCACGTGCATATACTCCTGCT | 59.44 | 58.97 | 260 | 72 |
100.00%
|
33.33%
|
| Primer 987 | Methanosphaera | AGTGCATACCTATGGGCATCA | TCACGTGCATATACTCCTGCT | 58.94 | 58.97 | 257 | 72 |
100.00%
|
33.33%
|
| Primer 988 | Methanosphaera | AACAAGTGCATACCTATGGGC | TCACGTGCATATACTCCTGCT | 58.35 | 58.97 | 261 | 72 |
100.00%
|
33.33%
|
| Primer 989 | Methanosphaera | GTGCATACCTATGGGCATCAA | TCACGTGCATATACTCCTGCT | 58.14 | 58.97 | 256 | 72 |
100.00%
|
33.33%
|
| Primer 990 | Methanosphaera | CAAGTGCATACCTATGGGCAT | TCACGTGCATATACTCCTGCT | 58.13 | 58.97 | 259 | 72 |
100.00%
|
33.33%
|