| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Methylococcus | ACAGGCCTCGGCTTCTTTC | AGCGAATCGATCACGGCT | 60.00 | 59.19 | 272 | 128 |
100.00%
|
100.00%
|
| Primer 42 | Methylococcus | TTCTTTCTCGGCTGGCTGG | AGCGAATCGATCACGGCT | 60.00 | 59.19 | 260 | 128 |
100.00%
|
100.00%
|
| Primer 43 | Methylococcus | TCGGCTTCTTTCTCGGCTG | AGCGAATCGATCACGGCT | 60.08 | 59.19 | 265 | 128 |
100.00%
|
100.00%
|
| Primer 44 | Methylococcus | GCAAACCTCTCGACGGGAA | TGGCGACAAAGGCGAGAA | 60.01 | 59.58 | 151 | 128 |
100.00%
|
100.00%
|
| Primer 45 | Methylococcus | GCCATTCACAGCGAAACCC | TGGCGACAAAGGCGAGAA | 59.79 | 59.58 | 199 | 128 |
100.00%
|
100.00%
|
| Primer 46 | Methylococcus | GGCCATTCACAGCGAAACC | TGGCGACAAAGGCGAGAA | 59.79 | 59.58 | 200 | 128 |
100.00%
|
100.00%
|
| Primer 47 | Methylococcus | TCACAGCGAAACCCAGGTG | TGGCGACAAAGGCGAGAA | 60.23 | 59.58 | 194 | 128 |
100.00%
|
100.00%
|
| Primer 48 | Methylococcus | TCACAGCGAAACCCAGGT | TGGCGACAAAGGCGAGAA | 58.76 | 59.58 | 194 | 128 |
100.00%
|
100.00%
|
| Primer 49 | Methylococcus | GCAACCAAGTTCGCAGTGG | TGAACATGCGGTCCAGCT | 60.01 | 59.25 | 250 | 128 |
100.00%
|
100.00%
|
| Primer 50 | Methylococcus | TGCCGTGTTCGAGCAACT | AGCGGTTCCAGCAGCAAT | 59.89 | 59.97 | 270 | 128 |
100.00%
|
100.00%
|