| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 171 | Methylorubrum | GCTCTTGCAGCCGAAGTT | ACATGACGTGCACGGTGT | 58.34 | 59.89 | 179 | 104 |
100.00%
|
25.00%
|
| Primer 172 | Methylorubrum | TCAACCATCCCCACGCT | TTCGTCGAACGTGCCCT | 58.04 | 58.86 | 243 | 104 |
100.00%
|
25.00%
|
| Primer 173 | Methylorubrum | TGGCGACCGTGAAAGTGA | GCGATCTTCACCTTGCCCT | 59.18 | 60.08 | 163 | 104 |
100.00%
|
50.00%
|
| Primer 174 | Methylorubrum | AAGCTCTCGAACGTCCTGC | CAGTTGACGTAGGTGCGGA | 60.08 | 59.71 | 205 | 104 |
100.00%
|
50.00%
|
| Primer 175 | Methylorubrum | AGGAATCGATGCTGCGCA | CGAGGATGATGCGGTTGGT | 59.81 | 60.15 | 294 | 103 |
100.00%
|
25.00%
|
| Primer 176 | Methylorubrum | TCGCGAATCGGCAGGTATC | TTGGTGCACGGTGTCGAA | 60.01 | 59.81 | 284 | 103 |
100.00%
|
25.00%
|
| Primer 177 | Methylorubrum | CCGCACATGAAGCAGAACG | TTGGTGCACGGTGTCGAA | 59.87 | 59.81 | 264 | 103 |
100.00%
|
25.00%
|
| Primer 178 | Methylorubrum | TGCCCTCAAAGCGCAGAA | GGACGTTCTTGGGCAGGAA | 59.89 | 59.93 | 296 | 103 |
100.00%
|
25.00%
|
| Primer 179 | Methylorubrum | TGCCCTCAAAGCGCAGAA | CCGCTATCGACGAGCTTGA | 59.89 | 59.64 | 222 | 103 |
100.00%
|
25.00%
|
| Primer 180 | Methylorubrum | TGCCCTCAAAGCGCAGAA | TCAGGACGTTCTTGGGCAG | 59.89 | 59.63 | 299 | 103 |
100.00%
|
25.00%
|