| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1961 | Methylorubrum | TCCCGATCGGAACCCTGAT | TTCCCACCAACAACGCGT | 60.08 | 60.12 | 151 | 62 |
100.00%
|
25.00%
|
| Primer 1962 | Methylorubrum | ATCCCGATCGGAACCCTGA | TTCCCACCAACAACGCGT | 60.08 | 60.12 | 152 | 62 |
100.00%
|
25.00%
|
| Primer 1963 | Methylorubrum | GCTCATGCAGATCCGCAAG | TTCCCACCAACAACGCGT | 59.35 | 60.12 | 264 | 62 |
100.00%
|
25.00%
|
| Primer 1964 | Methylorubrum | AAGGCGTTCGAAGGACTGC | TTCCCACCAACAACGCGT | 60.67 | 60.12 | 248 | 62 |
100.00%
|
25.00%
|
| Primer 1965 | Methylorubrum | GCAAGGCGTTCGAAGGACT | TTCCCACCAACAACGCGT | 60.67 | 60.12 | 250 | 62 |
100.00%
|
25.00%
|
| Primer 1966 | Methylorubrum | ACGGCTACATCCTCGGACA | GGCAGGTTGTAGACGAGCA | 60.38 | 59.71 | 178 | 62 |
100.00%
|
50.00%
|
| Primer 1967 | Methylorubrum | GGGCTGATGCTCCTCGAAA | TGGACGTTGGTGATCCAGC | 59.78 | 60.00 | 185 | 62 |
100.00%
|
50.00%
|
| Primer 1968 | Methylorubrum | GCCCTTGATGCGTTTCGAC | ATCTGCACCACGACCACA | 59.87 | 58.85 | 210 | 62 |
100.00%
|
25.00%
|
| Primer 1969 | Methylorubrum | TCTGCCTGATGCTGCACA | TAGCGCTTGGAGACCTTGG | 59.25 | 59.40 | 220 | 62 |
100.00%
|
50.00%
|
| Primer 1970 | Methylorubrum | AGCAGAGCCTCGACAACGT | CGGCATCCGCATGATCTCT | 61.57 | 60.01 | 188 | 62 |
100.00%
|
50.00%
|