| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2531 | Methylorubrum | TCACGCTGATGAACGCCT | GGAACCAATGCTCCAGGGT | 59.34 | 59.62 | 273 | 55 |
100.00%
|
50.00%
|
| Primer 2532 | Methylorubrum | ATTCTCGTGCTCTGGTGCC | CGGCAACATGCTTCGTGT | 60.08 | 59.05 | 276 | 55 |
100.00%
|
25.00%
|
| Primer 2533 | Methylorubrum | TTTCACCGTCGGCACCAA | CGATCGCCACGAGGATCAA | 59.81 | 59.93 | 230 | 55 |
100.00%
|
25.00%
|
| Primer 2534 | Methylorubrum | TTTCACCGTCGGCACCAA | GCAATTCATGACGGTGGCC | 59.81 | 59.86 | 271 | 55 |
100.00%
|
25.00%
|
| Primer 2535 | Methylorubrum | TTTCACCGTCGGCACCAA | GGCAATTCATGACGGTGGC | 59.81 | 59.86 | 272 | 55 |
100.00%
|
25.00%
|
| Primer 2536 | Methylorubrum | TCGACAAGCTCAACCGCT | GCTCGTAATGCTTGGCCAC | 59.27 | 59.57 | 213 | 55 |
100.00%
|
50.00%
|
| Primer 2537 | Methylorubrum | TACGCTCTGGGTCCGGTAT | GAGCGGACTTCGTTGGGAT | 59.77 | 59.78 | 240 | 55 |
100.00%
|
25.00%
|
| Primer 2538 | Methylorubrum | TACGCTCTGGGTCCGGTAT | AATAGCCCATCACGTCGGC | 59.77 | 60.23 | 182 | 55 |
100.00%
|
25.00%
|
| Primer 2539 | Methylorubrum | TACGCTCTGGGTCCGGTAT | AGCGGACTTCGTTGGGAT | 59.77 | 58.61 | 239 | 55 |
100.00%
|
25.00%
|
| Primer 2540 | Methylorubrum | ACCCTGCTCGACACCCATA | AGCTCGTCCTCGGCAAAT | 60.31 | 59.02 | 266 | 55 |
100.00%
|
25.00%
|