| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3301 | Methylorubrum | GCCGTTCTCCTCAACGTCT | CCGAGTTCGTGGAAACGGT | 59.71 | 60.30 | 257 | 45 |
100.00%
|
25.00%
|
| Primer 3302 | Methylorubrum | TCGATTCCCGACTACCCGA | TGCCGTATTCGAGCGCAT | 59.78 | 59.89 | 268 | 45 |
100.00%
|
25.00%
|
| Primer 3303 | Methylorubrum | GCCGAGAAGGAAGCGAAGT | CCGTGGACGCAGAATTCGA | 60.08 | 60.45 | 224 | 45 |
100.00%
|
50.00%
|
| Primer 3304 | Methylorubrum | AGCCTCGTCCACCTGATCT | CGATGACCTGGATCGCGAA | 60.00 | 59.93 | 166 | 45 |
100.00%
|
50.00%
|
| Primer 3305 | Methylorubrum | AAGCTGTCCTACCTCGCCT | GCCACGGTCTTCTTCACGA | 60.31 | 60.01 | 266 | 45 |
100.00%
|
50.00%
|
| Primer 3306 | Methylorubrum | TGCCGAAACAGACAACCGA | ACCCTCTTCGAAGAAGGCG | 59.86 | 59.41 | 221 | 45 |
100.00%
|
25.00%
|
| Primer 3307 | Methylorubrum | CCGCAATCGCTCTATCCGA | ACACGATCAGGTGGCTGAC | 59.71 | 59.71 | 205 | 45 |
100.00%
|
25.00%
|
| Primer 3308 | Methylorubrum | GTCGGTCTGCTTGTAGCGA | AATAGCGCTCCTGCTCGTC | 59.79 | 59.93 | 289 | 45 |
100.00%
|
25.00%
|
| Primer 3309 | Methylorubrum | TGATCCCGATACGCGTCGA | ATCGATTTCGGTCAGCGTCC | 60.89 | 60.53 | 296 | 45 |
100.00%
|
25.00%
|
| Primer 3310 | Methylorubrum | TGATCCCGATACGCGTCGA | ATCGATTTCGGTCAGCGTC | 60.89 | 58.04 | 296 | 45 |
100.00%
|
25.00%
|