| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 4141 | Methylorubrum | TGCACGGCATGGAATCGA | TCGTGTTCACCGATTGGGG | 59.73 | 60.00 | 294 | 9 |
100.00%
|
25.00%
|
| Primer 4142 | Methylorubrum | TTACAGCGTGCTCACCGA | TGAACACCTCGTTGGCCA | 58.96 | 59.09 | 251 | 9 |
100.00%
|
25.00%
|
| Primer 4143 | Methylorubrum | TGTTCTTCGACCACGCCA | CCGACTTCGATGTTGCCGA | 59.18 | 60.45 | 184 | 9 |
100.00%
|
25.00%
|
| Primer 4144 | Methylorubrum | TCTTCATCGACCCGACCCT | GGCACGTTGGTCAGATCGA | 60.00 | 60.08 | 235 | 9 |
100.00%
|
25.00%
|
| Primer 4145 | Methylorubrum | AGGCCTCGGTCTCGATCAT | TCGTTGAACTCGTTGGCGA | 60.15 | 59.93 | 163 | 9 |
100.00%
|
50.00%
|
| Primer 4146 | Methylorubrum | CCAACGGCTCGGTCGATTA | AGAGGAAATGCGTGCCGA | 59.86 | 59.34 | 189 | 0 |
100.00%
|
50.00%
|