| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 421 | Methylorubrum | ACGGCATGCTGTTCGACT | TGAGTTCGAGCACTGCGAG | 59.66 | 60.08 | 186 | 93 |
100.00%
|
50.00%
|
| Primer 422 | Methylorubrum | TACGCTCTGTTCGGCTTCC | CAGGAGATGCAGCGTCTGT | 59.79 | 59.78 | 201 | 93 |
100.00%
|
25.00%
|
| Primer 423 | Methylorubrum | ATGGTGGAGAAGCCCAAGC | AGCGCATGCCGTAGAACT | 60.00 | 59.42 | 181 | 93 |
100.00%
|
50.00%
|
| Primer 424 | Methylorubrum | TTCGCGATCTCACCGTCAC | CCGAACACGATCTGGAGCA | 60.15 | 59.78 | 223 | 93 |
100.00%
|
50.00%
|
| Primer 425 | Methylorubrum | ATCGTCATGCACAACCGC | TTGCCAGCTCTTGCCGAA | 58.82 | 59.89 | 159 | 92 |
100.00%
|
25.00%
|
| Primer 426 | Methylorubrum | CATCGTCATGCACAACCGC | TTGCCAGCTCTTGCCGAA | 60.23 | 59.89 | 160 | 92 |
100.00%
|
25.00%
|
| Primer 427 | Methylorubrum | AAGCCCTCTCGATCGCAGA | AGTTGTCGCTGGAGAAGGC | 60.75 | 60.00 | 194 | 92 |
100.00%
|
50.00%
|
| Primer 428 | Methylorubrum | CCTTCTGGACCATGCGACA | AGAGGTCCGTGCCGTAGAT | 59.70 | 60.08 | 156 | 92 |
100.00%
|
25.00%
|
| Primer 429 | Methylorubrum | TGCTCGGCTTCGACGAAA | AGGGAGACGTCGAGCTGAT | 59.66 | 60.08 | 172 | 92 |
100.00%
|
50.00%
|
| Primer 430 | Methylorubrum | TGCTCGGCTTCGACGAAA | GCGCACGACCAGTTGATTC | 59.66 | 59.87 | 197 | 92 |
100.00%
|
50.00%
|