| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 531 | Methylorubrum | TGCTCGCGATCATTGCCT | TCACGCAGATCAGGAAGCC | 59.81 | 59.78 | 264 | 89 |
100.00%
|
50.00%
|
| Primer 532 | Methylorubrum | TGCTCGCGATCATTGCCT | GCGAGACCACAATCAGGGT | 59.81 | 59.70 | 156 | 89 |
100.00%
|
50.00%
|
| Primer 533 | Methylorubrum | TGCTGAACATCCAGAGCCTT | TGCCGAAGCCGACATTGT | 59.31 | 59.97 | 285 | 89 |
100.00%
|
50.00%
|
| Primer 534 | Methylorubrum | TCCGATCTTTTCCGACCGC | TCCGTCAGTTCGAGCCAGA | 60.15 | 60.60 | 204 | 89 |
100.00%
|
25.00%
|
| Primer 535 | Methylorubrum | TTGCTCGATTTCGGCTGC | TGCGCTTGAGATCGGCTT | 58.82 | 59.73 | 262 | 89 |
100.00%
|
50.00%
|
| Primer 536 | Methylorubrum | GTTGCTCGATTTCGGCTGC | TGCGCTTGAGATCGGCTT | 60.23 | 59.73 | 263 | 89 |
100.00%
|
50.00%
|
| Primer 537 | Methylorubrum | TTCGGCTGCGAAATCGTC | TGCGCTTGAGATCGGCTT | 58.53 | 59.73 | 253 | 89 |
100.00%
|
50.00%
|
| Primer 538 | Methylorubrum | CCAACCGCTACGTCGTCTT | AAGGGCAGGTCCGCAAAA | 60.08 | 59.80 | 253 | 89 |
100.00%
|
25.00%
|
| Primer 539 | Methylorubrum | ACCAAGCCCTTCCACATGG | TTCGTGGCTCGTCAGCTT | 59.92 | 59.27 | 165 | 89 |
100.00%
|
50.00%
|
| Primer 540 | Methylorubrum | GTCACCAAGCCCTTCCACA | TTCGTGGCTCGTCAGCTT | 59.85 | 59.27 | 168 | 89 |
100.00%
|
50.00%
|