| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 401 | Methylotenera | AAGCCGATTACGCCAGCA | ATCCACCACGCGTGCAAT | 59.73 | 60.36 | 196 | 50 |
100.00%
|
50.00%
|
| Primer 402 | Methylotenera | AGCCGATTACGCCAGCAT | ATCCACCACGCGTGCAAT | 59.49 | 60.36 | 195 | 50 |
100.00%
|
50.00%
|
| Primer 403 | Methylotenera | ATCCGCATGCATGGTGCT | GCCAAAGAAACCGCTTGCA | 60.12 | 59.93 | 217 | 50 |
100.00%
|
50.00%
|
| Primer 404 | Methylotenera | ATCCGCATGCATGGTGCT | TGCCAAAGAAACCGCTTGC | 60.12 | 59.93 | 218 | 50 |
100.00%
|
50.00%
|
| Primer 405 | Methylotenera | AGTGTTCCGCATGAGCCA | AGCCACGCAGTTTACGGT | 59.25 | 59.58 | 202 | 50 |
100.00%
|
50.00%
|
| Primer 406 | Methylotenera | TGGTTTAGTGGCTGCGCT | AACAGCACAGCCGTGAGT | 59.57 | 59.49 | 216 | 50 |
100.00%
|
50.00%
|
| Primer 407 | Methylotenera | TGGTTTAGTGGCTGCGCT | AGAACAGCACAGCCGTGA | 59.57 | 59.18 | 218 | 50 |
100.00%
|
50.00%
|
| Primer 408 | Methylotenera | TGCAGAGTCGATTGCGCA | AGCGCTCACGTGGCAAAT | 60.05 | 60.67 | 212 | 50 |
100.00%
|
50.00%
|
| Primer 409 | Methylotenera | ACCGCTTCTGGCATTGTGA | TGGCCTGCGTATTTACCGAA | 59.93 | 59.75 | 158 | 50 |
100.00%
|
100.00%
|
| Primer 410 | Methylotenera | TAGCCACCGTTGCCGTATC | GGCACGCATCACTGGATCT | 59.86 | 60.15 | 229 | 49 |
100.00%
|
50.00%
|