| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Microvirga | ATGTCAGACGAGCTGGACC | TTGCCCTTCAACGACCCTG | 59.11 | 60.23 | 171 | 120 |
100.00%
|
100.00%
|
| Primer 2 | Microvirga | AGAATGGCGTGCTCGATCC | TTCTCGACGACCGTGTTGG | 60.23 | 60.01 | 190 | 113 |
100.00%
|
100.00%
|
| Primer 3 | Microvirga | TTGAACCCGATCGAGCAGG | ACGGACGCATATCCAGCTG | 59.78 | 59.93 | 161 | 108 |
100.00%
|
100.00%
|
| Primer 4 | Microvirga | ATCGATCCGCTGTTCCAGG | TTCCTGGATCGCGATGTGG | 59.56 | 59.86 | 261 | 108 |
100.00%
|
100.00%
|
| Primer 5 | Microvirga | GTCGATCGCGTGTTCGAGA | GCCTCGAACTTGAGCACCT | 60.23 | 60.00 | 170 | 107 |
100.00%
|
100.00%
|
| Primer 6 | Microvirga | ATCGATCCGCTGTTCCAGG | ATCGCGATGTGGGTCATGG | 59.56 | 60.23 | 254 | 104 |
100.00%
|
100.00%
|
| Primer 7 | Microvirga | TTCACGAACTCCAGGCCAC | CTGTTGCAGCGTATCGAGC | 59.93 | 59.37 | 185 | 100 |
100.00%
|
100.00%
|
| Primer 8 | Microvirga | GCTGAAATCGCTTGCGACC | TGTCCAAACACCCGTCCAG | 60.23 | 59.85 | 202 | 100 |
100.00%
|
100.00%
|
| Primer 9 | Microvirga | ACCCTCAAGAATGGCGTGC | TTCTCGACGACCGTGTTGG | 60.68 | 60.01 | 197 | 99 |
100.00%
|
100.00%
|
| Primer 10 | Microvirga | ATGCGCTGGTGATCGTCA | AGCATCAGATCGACCGACG | 59.42 | 59.64 | 196 | 97 |
100.00%
|
100.00%
|