| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1251 | Mitsuokella | TCAAGATGTGCGAGCGCT | TGGGCTGCACGCTGAAAA | 59.74 | 60.52 | 280 | 68 |
100.00%
|
33.33%
|
| Primer 1252 | Mitsuokella | GCGCAAGAACAACACGCT | ACTTTGCCTTGCCGTCCA | 59.67 | 59.81 | 161 | 68 |
100.00%
|
33.33%
|
| Primer 1253 | Mitsuokella | TCGCAGATCAGCTACGTGC | TGATCATCGCGTGCTGCA | 60.23 | 60.13 | 211 | 68 |
100.00%
|
66.67%
|
| Primer 1254 | Mitsuokella | AGGGCTTTGTCGTCGAGTC | TGATCATCGCGTGCTGCA | 59.71 | 60.13 | 168 | 68 |
100.00%
|
66.67%
|
| Primer 1255 | Mitsuokella | TATTGCGCTCGCCATGGT | TGGAAGTGGCTGTAGGGCT | 59.81 | 60.54 | 171 | 68 |
100.00%
|
33.33%
|
| Primer 1256 | Mitsuokella | ACGCCAAGACGAAGACCA | GTTCCTCGACTTCGACGGT | 58.87 | 59.42 | 168 | 68 |
100.00%
|
33.33%
|
| Primer 1257 | Mitsuokella | AGGTCCTCGAGAAGGCACT | AATGTGCGCTCAAAGGTGC | 59.92 | 59.71 | 277 | 68 |
100.00%
|
66.67%
|
| Primer 1258 | Mitsuokella | TGATCGGCTTCATGGGCA | CAGGAGAGCGACATTGGCA | 59.00 | 60.08 | 257 | 68 |
100.00%
|
33.33%
|
| Primer 1259 | Mitsuokella | ACCCGTTTGAGCACAGCA | GGCAACAACAGCAAAGGCA | 59.81 | 59.86 | 178 | 68 |
100.00%
|
66.67%
|
| Primer 1260 | Mitsuokella | ACCCGTTTGAGCACAGCA | AGGCAACAACAGCAAAGGC | 59.81 | 59.56 | 179 | 68 |
100.00%
|
66.67%
|