| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Mitsuokella | GGAGCATCAACACGAGCCA | TCGTCACAAAGGTGCGCA | 60.38 | 60.20 | 270 | 125 |
100.00%
|
66.67%
|
| Primer 22 | Mitsuokella | ATGGCTGGCGATGATCTGG | AGCAGGTCATCGGCAATCC | 59.93 | 60.15 | 198 | 124 |
100.00%
|
33.33%
|
| Primer 23 | Mitsuokella | AGGCAAGCAGGGCAAAGT | ACACGCGTTGCCTTCTTG | 59.80 | 58.98 | 192 | 123 |
100.00%
|
33.33%
|
| Primer 24 | Mitsuokella | ACGGCATCATACGGTCGTG | TTGCGCTGCTGCATGTTC | 60.23 | 59.74 | 250 | 123 |
100.00%
|
66.67%
|
| Primer 25 | Mitsuokella | GGTCAACTCCGTCTCGCAT | AGCGCTCGTTGCAGAAGT | 59.78 | 59.97 | 233 | 123 |
100.00%
|
66.67%
|
| Primer 26 | Mitsuokella | TCACGTCCGTCGAATGCA | CCTGGATGTGTGCGCTGTA | 59.35 | 60.08 | 213 | 123 |
100.00%
|
33.33%
|
| Primer 27 | Mitsuokella | TGGCAAGAACGTCGCTGA | TCTTGAACGCCTGTCCACG | 59.58 | 60.30 | 212 | 122 |
100.00%
|
66.67%
|
| Primer 28 | Mitsuokella | TTCGACCGCAGCCAGAAA | GAGGCTTCCTCGTGCATGA | 59.58 | 59.78 | 245 | 122 |
100.00%
|
66.67%
|
| Primer 29 | Mitsuokella | TCCTGCTCTATCGTCCGCA | AGCGCACGACTTCGATGA | 60.45 | 59.43 | 177 | 122 |
100.00%
|
66.67%
|
| Primer 30 | Mitsuokella | CTCGGTCCAATCGTCGTCA | AGCGCACGACTTCGATGA | 59.50 | 59.43 | 214 | 122 |
100.00%
|
33.33%
|