| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 331 | Mitsuokella | CTGACCCGTTTGCGCAAT | TGCTTTGCGTACTGGCGA | 59.05 | 59.97 | 242 | 99 |
100.00%
|
66.67%
|
| Primer 332 | Mitsuokella | ACTCGAAGCCAAACGCGA | GTCCGGATGTGCTCGAACA | 59.97 | 60.08 | 207 | 99 |
100.00%
|
33.33%
|
| Primer 333 | Mitsuokella | ACTCGAAGCCAAACGCGA | CAGCTGTCAATCGAGGCGA | 59.97 | 60.15 | 297 | 99 |
100.00%
|
33.33%
|
| Primer 334 | Mitsuokella | ACTCGAAGCCAAACGCGA | ATAGGCACTGCAGGTCAGC | 59.97 | 59.78 | 175 | 99 |
100.00%
|
33.33%
|
| Primer 335 | Mitsuokella | ACTCGAAGCCAAACGCGA | AGCTGTCAATCGAGGCGA | 59.97 | 58.71 | 296 | 99 |
100.00%
|
33.33%
|
| Primer 336 | Mitsuokella | ACTCGAAGCCAAACGCGA | TCTTGATGGCCTCTCCCGT | 59.97 | 60.31 | 224 | 99 |
100.00%
|
33.33%
|
| Primer 337 | Mitsuokella | ACTCGAAGCCAAACGCGA | GCGAAGTTGCTGATCTCGC | 59.97 | 59.65 | 246 | 99 |
100.00%
|
33.33%
|
| Primer 338 | Mitsuokella | TGTCGGCATCATCGCGAT | TGCTGTGCCTTGATGTCGA | 59.58 | 59.63 | 201 | 99 |
100.00%
|
66.67%
|
| Primer 339 | Mitsuokella | TCACGTCCGTCGAATGCA | TGTGTGCGCTGTAGTCGA | 59.35 | 58.96 | 207 | 99 |
100.00%
|
33.33%
|
| Primer 340 | Mitsuokella | TCATGGCCATCCCGTTCAC | AGCCGTAATCACTGCCCAC | 60.08 | 60.08 | 174 | 99 |
100.00%
|
33.33%
|