| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Mycoplasmopsis | GCAATGATGGCCAAATGTGC | AAGGGAGCCAAACATTCGCT | 58.99 | 60.25 | 239 | 95 |
100.00%
|
40.00%
|
| Primer 42 | Mycoplasmopsis | ACGACGAAGATAGAGCGCT | AGCCACAATGCCAGCTACT | 58.60 | 59.31 | 155 | 95 |
100.00%
|
40.00%
|
| Primer 43 | Mycoplasmopsis | TGCACGAAGGTCACATTGC | AGCAGAGCGCACAAGGAAA | 59.05 | 60.23 | 233 | 93 |
100.00%
|
40.00%
|
| Primer 44 | Mycoplasmopsis | TGCACGAAGGTCACATTGC | GCAGAGCGCACAAGGAAA | 59.05 | 58.66 | 232 | 93 |
100.00%
|
40.00%
|
| Primer 45 | Mycoplasmopsis | GCAATGATGGCCAAATGTGC | AGGGAGCCAAACATTCGCTT | 58.99 | 60.25 | 238 | 92 |
100.00%
|
40.00%
|
| Primer 46 | Mycoplasmopsis | GCAATGATGGCCAAATGTGC | GGGAGCCAAACATTCGCTT | 58.99 | 58.73 | 237 | 92 |
100.00%
|
40.00%
|
| Primer 47 | Mycoplasmopsis | GCAATGATGGCCAAATGTGC | AAGGGAGCCAAACATTCGC | 58.99 | 58.73 | 239 | 92 |
100.00%
|
40.00%
|
| Primer 48 | Mycoplasmopsis | TTCGTTCTAGCCAGGGAGC | TCCCGAACCTTCATTGCCA | 59.10 | 59.24 | 200 | 91 |
100.00%
|
40.00%
|
| Primer 49 | Mycoplasmopsis | TTCGTTCTAGCCAGGGAGC | TTCCCGAACCTTCATTGCCA | 59.10 | 59.89 | 201 | 91 |
100.00%
|
40.00%
|
| Primer 50 | Mycoplasmopsis | GGGTAATGGAGTTGTCGCCA | TGAACCAAGTGCTGCACCA | 60.04 | 60.08 | 280 | 91 |
100.00%
|
40.00%
|