| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1261 | Nostoc | TGGCACGTCAAGCAGTGA | ACGCCACAGCAACGGTTA | 59.50 | 59.89 | 186 | 27 |
100.00%
|
50.00%
|
| Primer 1262 | Nostoc | TGCCAAAGGTGCTGACGA | CGCCAAACTCGACCTGGAT | 59.49 | 60.08 | 246 | 27 |
100.00%
|
50.00%
|
| Primer 1263 | Nostoc | ACAGCCGTTGCAGCATCT | TCATCAACACGACCCGACG | 59.97 | 60.08 | 220 | 27 |
100.00%
|
50.00%
|
| Primer 1264 | Nostoc | AAGCAGTAACCCGTGCGA | ATGGGTTGGTCGCCTATGC | 59.26 | 60.15 | 175 | 27 |
100.00%
|
50.00%
|
| Primer 1265 | Nostoc | CCCCAAGTGCGAGCCATAA | TGCCACCAACGAAGCTGA | 60.08 | 59.49 | 264 | 25 |
100.00%
|
50.00%
|
| Primer 1266 | Nostoc | TCCCAACCACATTGCGGA | GGGGTTGTTCCTCGCGTAA | 59.16 | 60.00 | 245 | 25 |
100.00%
|
50.00%
|
| Primer 1267 | Nostoc | AATTCGGAGGCAAGCTGGG | TACGGGTTCCAATGCCACC | 60.38 | 60.00 | 195 | 24 |
100.00%
|
50.00%
|
| Primer 1268 | Nostoc | AGGGGATCGATGGTGTGGA | AGCAGACTCAAACCGTGGG | 60.00 | 59.93 | 151 | 24 |
100.00%
|
50.00%
|
| Primer 1269 | Nostoc | TGCGATCGCTGTGCTGTT | TGACCTGCAAACAACGCG | 60.36 | 59.29 | 228 | 22 |
100.00%
|
50.00%
|
| Primer 1270 | Nostoc | AAACGCCTTGCTGCCCTA | TTCGACCACCAGACAGCAC | 59.57 | 59.93 | 211 | 22 |
100.00%
|
50.00%
|