| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Parabacteroides | GAAAGCGGTGCTGTTGTGG | TGAACTGCTGACGGTGACC | 60.01 | 59.93 | 202 | 149 |
100.00%
|
64.29%
|
| Primer 2 | Parabacteroides | AAAGCGGTGCTGTTGTGG | TGAACTGCTGACGGTGACC | 58.88 | 59.93 | 201 | 149 |
100.00%
|
57.14%
|
| Primer 3 | Parabacteroides | GCAGAAAGCGGTGCTGTTG | TGAACTGCTGACGGTGACC | 60.37 | 59.93 | 205 | 131 |
100.00%
|
64.29%
|
| Primer 4 | Parabacteroides | AGCGGTGCTGTTGTTGAG | TGAACTGCTGACGGTGACC | 58.26 | 59.93 | 199 | 126 |
100.00%
|
50.00%
|
| Primer 5 | Parabacteroides | AAGCGGTGCTGTTGTTGAG | TGAACTGCTGACGGTGACC | 58.98 | 59.93 | 200 | 126 |
100.00%
|
64.29%
|
| Primer 6 | Parabacteroides | CAGAAAGCGGTGCTGTTGT | TGAACTGCTGACGGTGACC | 58.98 | 59.93 | 204 | 126 |
100.00%
|
64.29%
|
| Primer 7 | Parabacteroides | GCAGAAAGCGGTGCTGTT | TGAACTGCTGACGGTGACC | 58.97 | 59.93 | 205 | 122 |
100.00%
|
64.29%
|
| Primer 8 | Parabacteroides | AGCGGTGCTGTTGTTGAGT | TGAACTGCTGACGGTGACC | 60.15 | 59.93 | 199 | 121 |
100.00%
|
64.29%
|
| Primer 9 | Parabacteroides | GCGGTGCTGTTGTTGAGT | TGAACTGCTGACGGTGACC | 58.58 | 59.93 | 198 | 121 |
100.00%
|
50.00%
|
| Primer 10 | Parabacteroides | AAGCGGTGCTGTTGTGGA | TGAACTGCTGACGGTGACC | 59.81 | 59.93 | 200 | 118 |
100.00%
|
57.14%
|