| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Paraburkholderia | AACTCGCACAACTCGGCA | GAGCCTGCAGACCCTTGAT | 59.89 | 59.39 | 214 | 107 |
100.00%
|
66.67%
|
| Primer 2 | Paraburkholderia | AAACCGGTGGCAAGCAGT | TTTTGCGTGACGACCTTGC | 60.12 | 59.65 | 197 | 107 |
100.00%
|
66.67%
|
| Primer 3 | Paraburkholderia | TGTTCATGCACGCCACCA | CGTGACGCTGAAATGACCG | 60.20 | 59.58 | 155 | 104 |
100.00%
|
66.67%
|
| Primer 4 | Paraburkholderia | TGTTCATGCACGCCACCA | CATACACCATGCGATGCCG | 60.20 | 59.43 | 213 | 104 |
100.00%
|
66.67%
|
| Primer 5 | Paraburkholderia | TGTTCATGCACGCCACCA | GCCAGCGCATCATACACCA | 60.20 | 60.82 | 223 | 104 |
100.00%
|
66.67%
|
| Primer 6 | Paraburkholderia | TGCAGATTGGCTCGCACT | AGCCGTAGACGATGCGATC | 59.65 | 59.72 | 177 | 102 |
100.00%
|
66.67%
|
| Primer 7 | Paraburkholderia | AACTCGCACAACTCGGCA | AGACCCTTGATGGCTGCA | 59.89 | 58.50 | 206 | 102 |
100.00%
|
66.67%
|
| Primer 8 | Paraburkholderia | AGGCGAAATGCTGGCGAT | TGCCGAATACCGTGATGCA | 60.12 | 59.78 | 296 | 102 |
100.00%
|
66.67%
|
| Primer 9 | Paraburkholderia | AGCGTCGTTATGCCAGCA | AATCGTTCGCTGCGCAAC | 59.74 | 59.83 | 247 | 102 |
100.00%
|
66.67%
|
| Primer 10 | Paraburkholderia | ATGGATGGTGCGCTGCAT | TCAGCGTCGAAATCAGGCA | 60.12 | 59.71 | 296 | 100 |
100.00%
|
66.67%
|