| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Parascardovia | ACCCTCAACCGCATGCAT | TGGGAAATCCTGCGGTTGT | 59.64 | 59.54 | 212 | 148 |
100.00%
|
50.00%
|
| Primer 2 | Parascardovia | ATGTGAGCGTCACCACCAG | ATCGGACGTACATGCGGTC | 60.00 | 59.93 | 285 | 147 |
100.00%
|
50.00%
|
| Primer 3 | Parascardovia | TCACGTCCGTCAACGAGAC | TGGCGACCAAAGCGTCAT | 59.72 | 59.97 | 240 | 141 |
100.00%
|
50.00%
|
| Primer 4 | Parascardovia | ATCTCGTGACACTCGCAGC | AAGTGACGAAGCCCAGCA | 60.15 | 59.17 | 210 | 138 |
100.00%
|
50.00%
|
| Primer 5 | Parascardovia | ACGCTCGGGACCATAGACA | TGGCGACCAAAGCGTCAT | 60.38 | 59.97 | 262 | 137 |
100.00%
|
50.00%
|
| Primer 6 | Parascardovia | GGCAAGCACCGCAACAAA | AAACTCGTCGCTGTCGGA | 59.89 | 58.96 | 171 | 137 |
100.00%
|
100.00%
|
| Primer 7 | Parascardovia | GGCAAGCACCGCAACAAA | AACTCGTCGCTGTCGGAAG | 59.89 | 60.08 | 170 | 137 |
100.00%
|
100.00%
|
| Primer 8 | Parascardovia | TGTTTTGCGCACCGTCTG | ACCTTGCCGTCGATGTTGT | 59.29 | 59.93 | 165 | 137 |
100.00%
|
100.00%
|
| Primer 9 | Parascardovia | ACGCAATCAACGCCAAGC | TTCCCAGCATGATGGCCAG | 59.74 | 60.08 | 266 | 132 |
100.00%
|
100.00%
|
| Primer 10 | Parascardovia | GGCAAGCACCGCAACAAA | AACTCGTCGCTGTCGGAA | 59.89 | 58.96 | 170 | 128 |
100.00%
|
100.00%
|