| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Pediococcus | TTGCTCCAGCGCAACCAA | TCCACCACATTTCCGTCCG | 60.52 | 60.00 | 187 | 130 |
100.00%
|
40.00%
|
| Primer 22 | Pediococcus | TTGCTCCAGCGCAACCAA | TCGCCACTTCCACCACAT | 60.52 | 58.84 | 195 | 130 |
100.00%
|
40.00%
|
| Primer 23 | Pediococcus | TTGCTCCAGCGCAACCAA | ACAGCGACTGGACCTTTCC | 60.52 | 59.63 | 156 | 130 |
100.00%
|
40.00%
|
| Primer 24 | Pediococcus | GGCTTACCCGTCCTAATCGG | TGCCTCAAATCCCGTTGAAA | 59.97 | 58.01 | 209 | 130 |
100.00%
|
20.00%
|
| Primer 25 | Pediococcus | GGCTTACCCGTCCTAATCGG | TTGCCTCAAATCCCGTTGAAA | 59.97 | 58.69 | 210 | 130 |
100.00%
|
20.00%
|
| Primer 26 | Pediococcus | GGCTTACCCGTCCTAATCGG | AATTGCCTCAAATCCCGTTGA | 59.97 | 58.48 | 212 | 130 |
100.00%
|
20.00%
|
| Primer 27 | Pediococcus | GGTTCGGGAGTTTGTGGAAC | CGCACTCAAAACAGAACTGTC | 59.05 | 58.03 | 261 | 129 |
100.00%
|
40.00%
|
| Primer 28 | Pediococcus | TGCACCTGACGTGGTTGA | TTGGTGGCAACTTGGGGT | 59.10 | 59.31 | 216 | 128 |
100.00%
|
40.00%
|
| Primer 29 | Pediococcus | TGCTTGTGCTGGCAGTGT | CTGTCCCCAAACGTCTGCT | 60.12 | 59.93 | 194 | 128 |
100.00%
|
60.00%
|
| Primer 30 | Pediococcus | ACAGCAAAACGCACTGGT | ACACCGCTTGGCAAGTCA | 58.08 | 59.81 | 206 | 128 |
100.00%
|
80.00%
|