| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Phascolarctobacterium | GGTGCGCTTTCAGCATCTG | ACGCACTATCTGCTCCAGC | 59.86 | 59.86 | 163 | 134 |
100.00%
|
66.67%
|
| Primer 2 | Phascolarctobacterium | AGAATGCAGGAGCTGGCAG | TGCGCACTTTTGCTGCAG | 60.08 | 59.97 | 210 | 131 |
100.00%
|
66.67%
|
| Primer 3 | Phascolarctobacterium | CGTTCCGGAAACTACAGACAC | GCTGCGCACAAAACAACTG | 58.94 | 59.08 | 156 | 127 |
100.00%
|
66.67%
|
| Primer 4 | Phascolarctobacterium | CGTTCCGGAAACTACAGACAC | TCCAGATTAGAGCTGCGCAC | 58.94 | 60.18 | 167 | 127 |
100.00%
|
66.67%
|
| Primer 5 | Phascolarctobacterium | CGTTCCGGAAACTACAGACAC | CTGCGCACAAAACAACTGC | 58.94 | 59.08 | 155 | 126 |
100.00%
|
66.67%
|
| Primer 6 | Phascolarctobacterium | ACCGTGCCAAGACAGCAT | ATCCGCACCGTTTTGCAG | 59.57 | 59.05 | 260 | 124 |
100.00%
|
33.33%
|
| Primer 7 | Phascolarctobacterium | ACCGTGCCAAGACAGCAT | AGCCAAGGTAATCCGCACC | 59.57 | 60.08 | 270 | 124 |
100.00%
|
66.67%
|
| Primer 8 | Phascolarctobacterium | ACCGTGCCAAGACAGCAT | TAGCAGTGCCGAAGACAGC | 59.57 | 60.08 | 294 | 124 |
100.00%
|
66.67%
|
| Primer 9 | Phascolarctobacterium | ACGGCTCTTATGCTGGTGG | TAGCCTTGGCTGCTGTGTC | 59.78 | 60.00 | 157 | 116 |
100.00%
|
33.33%
|
| Primer 10 | Phascolarctobacterium | CCGCTAAGGTGAGCAATGC | CTTCGCTCTGCAGACGGAT | 59.28 | 59.86 | 195 | 116 |
100.00%
|
66.67%
|