| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Porphyromonas | TGGCCACGAGGGATGAAA | TGTGACGCTGAGTTCTCGG | 58.51 | 59.71 | 161 | 130 |
100.00%
|
36.36%
|
| Primer 2 | Porphyromonas | AGGCGATCGAGTACAGGTG | CGTGTAGCGCTTGAGGATAGG | 58.89 | 60.60 | 151 | 129 |
100.00%
|
27.27%
|
| Primer 3 | Porphyromonas | TACGCAACGTGCCCACAT | ACGCTTGAGTGTGACACCC | 59.97 | 60.23 | 242 | 129 |
100.00%
|
27.27%
|
| Primer 4 | Porphyromonas | ATCGTGCTGGGGTGCAAT | TCGCTTTGCCGTCTCCAT | 59.64 | 59.34 | 204 | 128 |
100.00%
|
27.27%
|
| Primer 5 | Porphyromonas | ATCGTGCTGGGGTGCAAT | GGTTTGCCCTGTCGCTTTG | 59.64 | 60.01 | 215 | 128 |
100.00%
|
27.27%
|
| Primer 6 | Porphyromonas | ATCGTGCTGGGGTGCAAT | CCATCATCACCTGTCGGCA | 59.64 | 59.78 | 190 | 128 |
100.00%
|
27.27%
|
| Primer 7 | Porphyromonas | ATCGTGCTGGGGTGCAAT | ATTGCCTCAACACGCTTGC | 59.64 | 59.71 | 155 | 128 |
100.00%
|
27.27%
|
| Primer 8 | Porphyromonas | ACATTCACCCCACAGCAGG | TAGCCTTGCAGAAGCGCA | 59.92 | 59.65 | 153 | 127 |
100.00%
|
27.27%
|
| Primer 9 | Porphyromonas | CGCGACCTGATGGATGTCA | AGCGAGCGCACATTGTCT | 59.86 | 60.05 | 248 | 127 |
100.00%
|
27.27%
|
| Primer 10 | Porphyromonas | ACAACCTGCACCGCATGT | TAGTGCTTGCCCTCCATGC | 60.20 | 60.08 | 211 | 126 |
100.00%
|
27.27%
|