| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Priestia | CGACGGCTGGTTCGATTTC | AAGCGCTTGCGCAATTCC | 59.29 | 60.13 | 275 | 133 |
100.00%
|
100.00%
|
| Primer 12 | Priestia | AAAAGCCGTCCTGCAGGA | TGCGCTTCTCTTTTGGAAGG | 59.16 | 58.76 | 158 | 130 |
100.00%
|
100.00%
|
| Primer 13 | Priestia | AAAAGCCGTCCTGCAGGA | TTGCGCTTCTCTTTTGGAAGG | 59.16 | 59.39 | 159 | 130 |
100.00%
|
100.00%
|
| Primer 14 | Priestia | AAAAGCCGTCCTGCAGGA | AGTTTGCGCTTCTCTTTTGGA | 59.16 | 58.70 | 162 | 130 |
100.00%
|
100.00%
|
| Primer 15 | Priestia | ACCGCACCCATTAACCTTGT | TCTTGAAACGCTTCTTCCGGA | 59.89 | 59.93 | 152 | 130 |
100.00%
|
100.00%
|
| Primer 16 | Priestia | ACCGCACCCATTAACCTTGT | CTTGAAACGCTTCTTCCGGA | 59.89 | 58.49 | 151 | 130 |
100.00%
|
100.00%
|
| Primer 17 | Priestia | GGAAGGCTGTATGGCATGC | CCGCTTCTTCGATTGCCTC | 58.96 | 58.99 | 265 | 130 |
100.00%
|
100.00%
|
| Primer 18 | Priestia | TGGTTTGAGACGCGAGCA | TGCGGTACAGGGTTCACAG | 59.58 | 59.63 | 202 | 128 |
100.00%
|
100.00%
|
| Primer 19 | Priestia | TGGTTTGAGACGCGAGCA | ACAGGGTTCACAGCCGAT | 59.58 | 58.52 | 196 | 128 |
100.00%
|
50.00%
|
| Primer 20 | Priestia | ACGCCACGTCTCGAGAAA | AGCATAGGAGTAAGGGTGCC | 58.96 | 58.87 | 169 | 127 |
100.00%
|
100.00%
|