| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Pseudescherichia | AAAGCTGCACTGCCAAGC | AACAGCAGGCCAATCAGC | 59.27 | 58.31 | 173 | 154 |
100.00%
|
100.00%
|
| Primer 2 | Pseudescherichia | TCAAATCCCCAGCCAGCAG | TCGTGTTGGGTAGCAGCTG | 60.00 | 60.01 | 206 | 148 |
100.00%
|
100.00%
|
| Primer 3 | Pseudescherichia | TGATGTCTCGAAGGCAGGC | ACCAAACGCCAGCTCGAT | 59.78 | 59.65 | 208 | 141 |
100.00%
|
100.00%
|
| Primer 4 | Pseudescherichia | ATGTGCGAACCCGAGTGT | TATCCGCATGGTGCAGCA | 59.26 | 59.41 | 214 | 138 |
100.00%
|
100.00%
|
| Primer 5 | Pseudescherichia | GGCAACGCGTTTGAGCAT | TTACGCAGTTCGCCATGC | 59.74 | 58.83 | 260 | 138 |
100.00%
|
100.00%
|
| Primer 6 | Pseudescherichia | GGCAACGCGTTTGAGCAT | GTTACGCAGTTCGCCATGC | 59.74 | 60.23 | 261 | 138 |
100.00%
|
100.00%
|
| Primer 7 | Pseudescherichia | AAGCGATGTTGACCCCAGG | TGCTCCCACGTTTTGGCA | 60.00 | 60.12 | 253 | 138 |
100.00%
|
100.00%
|
| Primer 8 | Pseudescherichia | TCATTGCGCTGTACGGCA | AGGCCAACGAAGAGCTGAC | 60.05 | 60.00 | 184 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Pseudescherichia | AGTTCGGTCGTGGCGAAA | GCAGTGTAGCGTGCAACA | 59.58 | 58.67 | 199 | 137 |
100.00%
|
100.00%
|
| Primer 10 | Pseudescherichia | ATTCGCGACCATTGGCCA | AGCAGTAAACCGGATGGGC | 60.05 | 60.08 | 293 | 135 |
100.00%
|
100.00%
|