| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Pseudescherichia | TTGTTGCCAGCATGACGC | GCTGCGCATCGGTATTGTG | 59.36 | 60.01 | 156 | 125 |
100.00%
|
100.00%
|
| Primer 42 | Pseudescherichia | GTCTGGGCATGAGCGAAGA | TGAGCAAACGCGTCGAGA | 59.78 | 59.67 | 201 | 125 |
100.00%
|
100.00%
|
| Primer 43 | Pseudescherichia | GCTATGTTCCGTCTGGGCA | TGAGCAAACGCGTCGAGA | 59.78 | 59.67 | 211 | 125 |
100.00%
|
100.00%
|
| Primer 44 | Pseudescherichia | TGTTCCGTCTGGGCATGAG | TGAGCAAACGCGTCGAGA | 59.70 | 59.67 | 207 | 125 |
100.00%
|
100.00%
|
| Primer 45 | Pseudescherichia | TGTTCCGTCTGGGCATGA | TGAGCAAACGCGTCGAGA | 58.52 | 59.67 | 207 | 125 |
100.00%
|
100.00%
|
| Primer 46 | Pseudescherichia | CTGGGCATGAGCGAAGAGA | TGAGCAAACGCGTCGAGA | 59.48 | 59.67 | 199 | 125 |
100.00%
|
100.00%
|
| Primer 47 | Pseudescherichia | TCTGGGCATGAGCGAAGAG | TGAGCAAACGCGTCGAGA | 59.48 | 59.67 | 200 | 125 |
100.00%
|
100.00%
|
| Primer 48 | Pseudescherichia | ATCCACCTGCGCTTCGAT | TGCGACTTGGTTGCCAGA | 59.10 | 59.49 | 263 | 125 |
100.00%
|
100.00%
|
| Primer 49 | Pseudescherichia | GATCCACCTGCGCTTCGAT | TGCGACTTGGTTGCCAGA | 60.23 | 59.49 | 264 | 125 |
100.00%
|
100.00%
|
| Primer 50 | Pseudescherichia | TGGCACGCGTTCTTGAGA | AAAACCGTGCGGAATGCC | 59.58 | 59.35 | 266 | 125 |
100.00%
|
100.00%
|