| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 201 | Pseudomonas | TGTTCGAGGACATGCGCA | AGGATACCGGTCTTGCCCT | 59.66 | 60.00 | 193 | 62 |
93.33%
|
41.18%
|
| Primer 202 | Pseudomonas | CCAACCTCAAGCAGCACCT | CCTTGGTCTGGTTGCCCTT | 60.23 | 59.85 | 198 | 62 |
92.86%
|
38.24%
|
| Primer 203 | Pseudomonas | TTCCCAGGCAAGGTCATCG | AGCCTTGCATCATGCCGT | 59.70 | 60.04 | 172 | 62 |
100.00%
|
41.18%
|
| Primer 204 | Pseudomonas | TGCAGGGCATTACCGTCA | TGTTGGCAGCCTTGGTGA | 58.93 | 59.40 | 174 | 62 |
100.00%
|
26.47%
|
| Primer 205 | Pseudomonas | AACCGCTCGCGTATTCCT | ACGCAGGGTTTCGTCGTA | 59.11 | 58.96 | 241 | 62 |
100.00%
|
26.47%
|
| Primer 206 | Pseudomonas | AACCGCTCGCGTATTCCT | ACGCAGGGTTTCGTCGTAG | 59.11 | 60.08 | 241 | 62 |
100.00%
|
26.47%
|
| Primer 207 | Pseudomonas | AACACCCGTGAGCGACAA | CCTTCGTCTGGTTGCCCTT | 59.50 | 59.93 | 218 | 61 |
100.00%
|
52.94%
|
| Primer 208 | Pseudomonas | TGTTCTCCCAGCGCAACT | CCGGTGGCCTTGATCTTCA | 59.17 | 59.70 | 220 | 61 |
100.00%
|
38.24%
|
| Primer 209 | Pseudomonas | ACATCCGCAACCTGCTCA | TCCATGCTGGTCAGGAGGA | 59.25 | 59.92 | 274 | 61 |
93.75%
|
44.12%
|
| Primer 210 | Pseudomonas | TGTTCTCCCAGCGCAACT | GCCTTGACCTTGACCAGCA | 59.17 | 60.23 | 214 | 61 |
94.12%
|
47.06%
|