| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 111 | Ralstonia | GTTTGCGCTGATTGCCGT | AAACCCAGGGCAAACGCT | 59.74 | 60.12 | 222 | 112 |
100.00%
|
33.33%
|
| Primer 112 | Ralstonia | TGCCGTTTGCCTACGACA | GGCAGCAGCGACAGATACA | 59.58 | 60.15 | 202 | 112 |
100.00%
|
16.67%
|
| Primer 113 | Ralstonia | ATTTCGGCACCTGCGACA | TGCCGATCAGCTTCACCAG | 59.97 | 60.08 | 243 | 112 |
100.00%
|
16.67%
|
| Primer 114 | Ralstonia | ATTTCGGCACCTGCGACA | ACACGACCTTGCCGATCAG | 59.97 | 60.08 | 252 | 112 |
100.00%
|
16.67%
|
| Primer 115 | Ralstonia | TTTGCGCACGCGAGACTA | ATGGTGCTGCCCAAAGGT | 60.05 | 59.47 | 229 | 111 |
100.00%
|
16.67%
|
| Primer 116 | Ralstonia | TCACGCAGCTCAACCAGT | ACTCGATCACCTTGGGCAC | 59.18 | 59.70 | 216 | 110 |
100.00%
|
16.67%
|
| Primer 117 | Ralstonia | TGCTGGCGTTCCTGTTCA | ACAGCACCGTGAACCACA | 59.49 | 59.41 | 249 | 110 |
100.00%
|
16.67%
|
| Primer 118 | Ralstonia | TGCTGGCGTTCCTGTTCA | ACAGCATCGTGTACGCCA | 59.49 | 59.35 | 198 | 110 |
100.00%
|
33.33%
|
| Primer 119 | Ralstonia | TGCTGGCGTTCCTGTTCA | TCGCGAAGTACGACAGCA | 59.49 | 59.05 | 210 | 110 |
100.00%
|
33.33%
|
| Primer 120 | Ralstonia | TGGCGTTCACGGTCATCA | GTGCGGATCAACGAACTGC | 59.26 | 59.87 | 236 | 110 |
100.00%
|
16.67%
|