| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1231 | Ralstonia | AGCTCAAGCAACTCGGCA | TGTTCAGCGTGGCGATCA | 59.57 | 59.66 | 216 | 54 |
100.00%
|
16.67%
|
| Primer 1232 | Ralstonia | TCGGCAAATCCACGCTGA | ACCACGAACAACACGGCA | 59.65 | 60.13 | 232 | 54 |
100.00%
|
16.67%
|
| Primer 1233 | Ralstonia | GCCGATGTGGTGACCATGA | ACGAACGCTGAGCACCAT | 60.08 | 59.66 | 202 | 54 |
100.00%
|
16.67%
|
| Primer 1234 | Ralstonia | TCCTGGTTGAACGCAGCA | AGCAGACGCGTGACTTCA | 59.49 | 59.27 | 211 | 54 |
100.00%
|
16.67%
|
| Primer 1235 | Ralstonia | TGTTCCTGCAAGTGGCCA | TTGGCATGCGCTCCTTGA | 59.40 | 59.97 | 273 | 54 |
100.00%
|
16.67%
|
| Primer 1236 | Ralstonia | TCGTCGCAGCCAAGATGA | TTGGCGTCGATGCTGTCA | 59.03 | 59.66 | 241 | 54 |
100.00%
|
33.33%
|
| Primer 1237 | Ralstonia | TGAAGATCGTCGCAGCCA | TTGGCGTCGATGCTGTCA | 59.03 | 59.66 | 247 | 54 |
100.00%
|
16.67%
|
| Primer 1238 | Ralstonia | TTCGTGCGCATCGTCAAC | CGGCTGTTCTGCTGGATGA | 59.15 | 60.08 | 284 | 54 |
100.00%
|
33.33%
|
| Primer 1239 | Ralstonia | ACGATCCTGACGGGTCTGA | TCATTGCTCGCGAACCCA | 60.00 | 59.65 | 189 | 54 |
100.00%
|
16.67%
|
| Primer 1240 | Ralstonia | TTCCCACCGCATCGTTCA | AAGGCCATGCACCACCAT | 59.26 | 59.55 | 163 | 54 |
100.00%
|
33.33%
|