| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1421 | Ralstonia | AAACTCGCTGCGCAATCG | TGGGGTTTGCGCTTTTGC | 59.52 | 59.89 | 176 | 46 |
100.00%
|
16.67%
|
| Primer 1422 | Ralstonia | AACCACGTGCAATTGCCG | TGCCCTTCAAGTTCCGCAT | 59.67 | 59.93 | 152 | 46 |
100.00%
|
16.67%
|
| Primer 1423 | Ralstonia | TCTATCCGCACAACGCCA | CGTCCCACCATTCCTGCAT | 59.03 | 60.08 | 222 | 46 |
100.00%
|
16.67%
|
| Primer 1424 | Ralstonia | GCACGTGAGCTTTGCCAT | TTTGCGCACGTCGTACCA | 59.04 | 60.28 | 217 | 46 |
100.00%
|
33.33%
|
| Primer 1425 | Ralstonia | ACCAGCTTGCCATCACCA | TGCTGGATGCCGTTCACA | 59.15 | 59.57 | 154 | 46 |
100.00%
|
16.67%
|
| Primer 1426 | Ralstonia | GCAGCGCTGGATTCAATCG | TTGCGTGTTGCTGCCAAG | 60.01 | 59.59 | 261 | 46 |
100.00%
|
16.67%
|
| Primer 1427 | Ralstonia | ACGACACCCTTGCCGAAA | ACCGTTGGTGTTGAGCCA | 59.49 | 59.41 | 158 | 46 |
100.00%
|
16.67%
|
| Primer 1428 | Ralstonia | AAAGCCATCGTGCATGCG | TGCACCTGCGCTCATCTT | 59.51 | 59.65 | 285 | 46 |
100.00%
|
33.33%
|
| Primer 1429 | Ralstonia | TGTTTTCGCAGGTGGCCA | CGTTGCCACAACGACAAGG | 60.12 | 60.01 | 203 | 46 |
100.00%
|
16.67%
|
| Primer 1430 | Ralstonia | GTTACCAGCCAGTGCCCAT | ACCTTGCTTGGCTTGCCA | 60.00 | 60.12 | 248 | 46 |
100.00%
|
16.67%
|