| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 591 | Ralstonia | TTCGGAATGGCAGGGGTT | CAGACCATGAGCTGCACCA | 58.82 | 60.00 | 219 | 78 |
100.00%
|
16.67%
|
| Primer 592 | Ralstonia | TGCCGTTCGTGCATGTGT | TGAACAGCGCATCGGGTT | 60.59 | 59.97 | 180 | 78 |
100.00%
|
16.67%
|
| Primer 593 | Ralstonia | TCGAATTCCACACCAGCCC | CGATGTTGTGCGAGTCCCT | 60.00 | 60.08 | 234 | 78 |
100.00%
|
16.67%
|
| Primer 594 | Ralstonia | CCAAGAACGACCATCGCCT | GCGCTTTTGCTTGCCCTT | 60.08 | 59.97 | 206 | 78 |
100.00%
|
33.33%
|
| Primer 595 | Ralstonia | TGCGCTGACGTTCGAGTT | AGCATCAAGACCAGCCCAC | 59.97 | 60.00 | 240 | 78 |
100.00%
|
33.33%
|
| Primer 596 | Ralstonia | TGCGCTGACGTTCGAGTT | ACCAGCATCAAGACCAGCC | 59.97 | 60.00 | 243 | 78 |
100.00%
|
33.33%
|
| Primer 597 | Ralstonia | TGCGCTGACGTTCGAGTT | CAGCATCAAGACCAGCCCA | 59.97 | 60.00 | 241 | 78 |
100.00%
|
33.33%
|
| Primer 598 | Ralstonia | TGCAATTGACGGACGCCA | CGGTCATGCAGATACGCCT | 60.28 | 59.93 | 175 | 78 |
100.00%
|
16.67%
|
| Primer 599 | Ralstonia | TGCGCTCATCACCTCGTT | TTGCATCGAGCTGCGTCA | 59.34 | 60.05 | 245 | 78 |
100.00%
|
33.33%
|
| Primer 600 | Ralstonia | ATGCGCTCATCACCTCGTT | TTGCATCGAGCTGCGTCA | 59.78 | 60.05 | 246 | 78 |
100.00%
|
33.33%
|