| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 621 | Ralstonia | ACATGGACATGGTGCGCA | TGCCACGCACCTTGATCA | 59.97 | 59.57 | 183 | 76 |
100.00%
|
16.67%
|
| Primer 622 | Ralstonia | ATGTTTGCGCTCACCACG | ACGCTTCGAACACCACCA | 59.05 | 59.50 | 211 | 76 |
100.00%
|
16.67%
|
| Primer 623 | Ralstonia | TTGCACGCGAGTTCGACA | TCTTCCAACTGCTGCGTCA | 60.28 | 59.55 | 286 | 76 |
100.00%
|
33.33%
|
| Primer 624 | Ralstonia | TCACGCAGCTCAACCAGT | ATTGCTGGTAGTCCGCACG | 59.18 | 60.45 | 165 | 76 |
100.00%
|
16.67%
|
| Primer 625 | Ralstonia | CTGAACGTCACCCTCAACGA | AATGCGGTCGCCTGTCTT | 59.97 | 59.65 | 180 | 76 |
100.00%
|
16.67%
|
| Primer 626 | Ralstonia | TCTCGCTCGCAATCTCGT | ATGATGGCGTTGCTGCGA | 58.81 | 60.44 | 256 | 76 |
100.00%
|
16.67%
|
| Primer 627 | Ralstonia | TCGCTCGCAATCTCGTTCA | ATGATGGCGTTGCTGCGA | 59.79 | 60.44 | 254 | 76 |
100.00%
|
16.67%
|
| Primer 628 | Ralstonia | GGCAAGCAACTGGTGGAGA | ATGATGGCGTTGCTGCGA | 60.23 | 60.44 | 176 | 76 |
100.00%
|
33.33%
|
| Primer 629 | Ralstonia | TCTCGCTCGCAATCTCGTT | ATGATGGCGTTGCTGCGA | 59.49 | 60.44 | 256 | 76 |
100.00%
|
16.67%
|
| Primer 630 | Ralstonia | TCGGAATGGCAGGGGTTT | CAGACCATGAGCTGCACCA | 58.82 | 60.00 | 218 | 76 |
100.00%
|
16.67%
|