| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Rhodothermus | ACTGGCCTTTTCCGCTCA | AACGATGCGCTGTGAGGT | 59.16 | 59.66 | 218 | 78 |
100.00%
|
100.00%
|
| Primer 2 | Rhodothermus | GACTGGCCTTTTCCGCTCA | AACGATGCGCTGTGAGGT | 60.30 | 59.66 | 219 | 71 |
100.00%
|
100.00%
|
| Primer 3 | Rhodothermus | ACTGGCCTTTTCCGCTCAT | AACGATGCGCTGTGAGGT | 59.62 | 59.66 | 218 | 71 |
100.00%
|
100.00%
|
| Primer 4 | Rhodothermus | ATACGACATCGCTGAGCCG | TTCCAGGTCCACGATGTGC | 60.01 | 60.00 | 154 | 56 |
100.00%
|
100.00%
|
| Primer 5 | Rhodothermus | ACCACGCACAACCTGATCC | AGCCGATACTCCTGCAGGA | 60.30 | 60.08 | 245 | 54 |
100.00%
|
100.00%
|
| Primer 6 | Rhodothermus | ACTGGCCTTTTCCGCTCA | TCCTCAACGATGCGCTGT | 59.16 | 59.34 | 223 | 46 |
100.00%
|
100.00%
|
| Primer 7 | Rhodothermus | CCACGCACAACCTGATCCT | AGCCGATACTCCTGCAGGA | 60.00 | 60.08 | 244 | 43 |
100.00%
|
100.00%
|
| Primer 8 | Rhodothermus | AGCGTGTTTCTGTGCCGA | CGATCCAGTGATCCTGTCGG | 59.89 | 59.97 | 181 | 16 |
100.00%
|
100.00%
|