| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Rickettsia | CCGACTTCTAGGGAACAGGC | CCCCAAGGTAATGGCTGACC | 59.82 | 60.40 | 155 | 133 |
100.00%
|
69.23%
|
| Primer 22 | Rickettsia | GCAGACGGGTTTGGTCGTA | ACCGAACCGAAAAGTTTACCA | 60.01 | 58.08 | 215 | 133 |
100.00%
|
53.85%
|
| Primer 23 | Rickettsia | GGTCTTGGTAGTAGGTCGGC | TACCGCTACGCTCCATTGC | 59.54 | 60.23 | 241 | 132 |
100.00%
|
61.54%
|
| Primer 24 | Rickettsia | ACGGTGTCGGTTATAGGGC | AGGCTCAGGTGGTCTCTGT | 59.18 | 59.84 | 200 | 132 |
100.00%
|
69.23%
|
| Primer 25 | Rickettsia | CCGACTTCTAGGGAACAGGC | TCCCCAAGGTAATGGCTGAC | 59.82 | 59.38 | 156 | 131 |
100.00%
|
69.23%
|
| Primer 26 | Rickettsia | AGCAGAATTTAAACCGCTGGA | TCATAGCCTCAGCGTGTGC | 58.21 | 60.15 | 167 | 130 |
100.00%
|
69.23%
|
| Primer 27 | Rickettsia | AGCAGAATTTAAACCGCTGGA | GCGTGTGCTTGTTCAAGC | 58.21 | 58.09 | 156 | 130 |
100.00%
|
61.54%
|
| Primer 28 | Rickettsia | AGCAGAATTTAAACCGCTGGA | ACTCATAGCCTCAGCGTGTG | 58.21 | 59.83 | 169 | 130 |
100.00%
|
69.23%
|
| Primer 29 | Rickettsia | AGCAGAATTTAAACCGCTGGA | ACTCATAGCCTCAGCGTGT | 58.21 | 58.42 | 169 | 130 |
100.00%
|
69.23%
|
| Primer 30 | Rickettsia | AGCAGAATTTAAACCGCTGGA | CTCATAGCCTCAGCGTGTG | 58.21 | 58.03 | 168 | 130 |
100.00%
|
69.23%
|