| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Roseburia | TGCACAGAAGGCAGCAGT | TGAAGCGCATTCACCTGC | 59.49 | 58.73 | 208 | 132 |
100.00%
|
50.00%
|
| Primer 2 | Roseburia | ACGCAATTATCGCAACAGGTG | GTAAACGCCTGTCTGTGGC | 59.87 | 59.13 | 271 | 132 |
100.00%
|
75.00%
|
| Primer 3 | Roseburia | TCGCAACAGGTGGTAAACAGT | GTAAACGCCTGTCTGTGGC | 60.13 | 59.13 | 262 | 132 |
100.00%
|
75.00%
|
| Primer 4 | Roseburia | CGCAACAGGTGGTAAACAGT | GTAAACGCCTGTCTGTGGC | 58.69 | 59.13 | 261 | 132 |
100.00%
|
75.00%
|
| Primer 5 | Roseburia | TGCGGATCTGCAGGCAAA | GTCCCCTGCGGTCAAGAAA | 59.97 | 59.93 | 185 | 126 |
100.00%
|
50.00%
|
| Primer 6 | Roseburia | TGTTCGGCTATCGGTGCAG | TCGTCTCTGCAACTGCCTG | 60.15 | 60.01 | 163 | 125 |
100.00%
|
50.00%
|
| Primer 7 | Roseburia | TACAGGCAGCAGCAGGTTC | ACGGCATCTGACCACCTTC | 60.00 | 59.70 | 153 | 123 |
100.00%
|
50.00%
|
| Primer 8 | Roseburia | TGCTGAAGGTACTGCGCT | AGGCTGTTTTCGGGTGCT | 58.94 | 59.48 | 168 | 118 |
100.00%
|
50.00%
|
| Primer 9 | Roseburia | CTGCTGAAGGTACTGCGCT | AGGCTGTTTTCGGGTGCT | 60.08 | 59.48 | 169 | 118 |
100.00%
|
50.00%
|
| Primer 10 | Roseburia | TGAAGGTACTGCGCTCTGC | AGGCTGTTTTCGGGTGCT | 60.08 | 59.48 | 165 | 118 |
100.00%
|
50.00%
|