| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Rothia | TGCGCAGTTGCTGAAGGA | CACCGAGCACGAACTCAGT | 59.89 | 60.01 | 251 | 140 |
100.00%
|
100.00%
|
| Primer 2 | Rothia | TGCGCAGTTGCTGAAGGA | TCACCGAGCACGAACTCAG | 59.89 | 59.71 | 252 | 140 |
100.00%
|
100.00%
|
| Primer 3 | Rothia | TGCGCAGTTGCTGAAGGA | ATTGACGCTACGGGAAGCC | 59.89 | 60.15 | 286 | 138 |
100.00%
|
100.00%
|
| Primer 4 | Rothia | TGCGACATTGGCTGAGCA | TGCACTCTTCCAAACGCCA | 59.97 | 60.15 | 233 | 135 |
100.00%
|
100.00%
|
| Primer 5 | Rothia | CCGAAGCAGAAGACCCACT | ACAACCTTTGCCTGGCCA | 59.33 | 59.72 | 170 | 135 |
100.00%
|
133.33%
|
| Primer 6 | Rothia | AGAAGACCCACTCTGGTGC | ACAACCTTTGCCTGGCCA | 58.94 | 59.72 | 163 | 135 |
100.00%
|
133.33%
|
| Primer 7 | Rothia | GCATCGCTGGCACTCATTG | TCTTCGTCGAGGATTGCCG | 59.94 | 59.86 | 257 | 135 |
100.00%
|
100.00%
|
| Primer 8 | Rothia | GGCACAGTTCGTCGTCGAT | GCAGCGTTCGCGTTTTCA | 60.44 | 60.05 | 273 | 132 |
100.00%
|
66.67%
|
| Primer 9 | Rothia | TGCGCAGTTGCTGAAGGA | GTCACCGAGCACGAACTCA | 59.89 | 60.01 | 253 | 132 |
100.00%
|
100.00%
|
| Primer 10 | Rothia | GGTTCACCCAGCAACCTGT | TGGGGTTTGCTGGCTTGT | 60.15 | 59.72 | 176 | 130 |
100.00%
|
66.67%
|