| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Rothia | TTCACCCAGCAACCTGTGG | TGGGGTTTGCTGGCTTGT | 60.15 | 59.72 | 174 | 130 |
100.00%
|
18.18%
|
| Primer 12 | Rothia | ATAGCCCAAGCGCTGTCTG | TGGGGTTTGCTGGCTTGT | 60.15 | 59.72 | 245 | 130 |
100.00%
|
18.18%
|
| Primer 13 | Rothia | TCGTAACGAGCAGCGCAA | TCTGGTCACGGTTGCGAAC | 60.05 | 60.59 | 173 | 130 |
100.00%
|
18.18%
|
| Primer 14 | Rothia | CGGTTCTCGTGTCTTGGCA | AGGCCATGCTTGCAGGAA | 60.30 | 59.56 | 194 | 128 |
100.00%
|
18.18%
|
| Primer 15 | Rothia | GCAGTTTCACGCATCGCT | AGATGGTGGCAACGGCAA | 59.14 | 59.88 | 236 | 128 |
100.00%
|
18.18%
|
| Primer 16 | Rothia | AGCTCGCGATGCAAAGCT | TGCCGTTACCGTCGAACT | 60.44 | 58.96 | 251 | 128 |
100.00%
|
27.27%
|
| Primer 17 | Rothia | AGCTCGCGATGCAAAGCT | TGGAACATGGACTGCTCGG | 60.44 | 59.70 | 288 | 128 |
100.00%
|
45.45%
|
| Primer 18 | Rothia | TTCGCGTGCATCACTGGT | AATCGCTCACCAGCTGCA | 59.97 | 59.65 | 152 | 128 |
100.00%
|
18.18%
|
| Primer 19 | Rothia | AAGAGGTTTCGCAGCGCT | AGCCTGATCCTGTGCGAT | 59.97 | 58.36 | 242 | 128 |
100.00%
|
18.18%
|
| Primer 20 | Rothia | AAGAATGCACGGCTGGCA | GCTTCTTCTTGGCGAGGGT | 60.28 | 60.00 | 172 | 128 |
100.00%
|
18.18%
|