| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 111 | Rothia | AGCCACTATTGCAAGCCGT | AACCAGCGAGTCAAGAGCC | 60.00 | 60.00 | 232 | 112 |
100.00%
|
36.36%
|
| Primer 112 | Rothia | AAGATCGAGCAGGCTGAGC | ATTGCCAGAACGTCGCGT | 59.86 | 60.36 | 242 | 112 |
100.00%
|
36.36%
|
| Primer 113 | Rothia | TGGATTGGCGTAGTCGTGC | ACGCTCGTCTTCACTGGTG | 60.45 | 60.01 | 203 | 112 |
100.00%
|
27.27%
|
| Primer 114 | Rothia | CGCCTGCGCAACAACATT | GCAGCAAGACCGGACTTCT | 60.05 | 60.00 | 191 | 112 |
100.00%
|
18.18%
|
| Primer 115 | Rothia | TTCGCGTGCATCACTGGT | TCCTCATCCACGGCACCTA | 59.97 | 60.00 | 297 | 111 |
100.00%
|
18.18%
|
| Primer 116 | Rothia | TTCGCGTGCATCACTGGT | TCATCCACGGCACCTACCT | 59.97 | 60.31 | 294 | 111 |
100.00%
|
18.18%
|
| Primer 117 | Rothia | ATCGCGTGCTGTTCACCT | ACGCACAGTACCTTCGCA | 59.66 | 59.27 | 199 | 111 |
100.00%
|
18.18%
|
| Primer 118 | Rothia | ATCGCGTGCTGTTCACCT | AGTACCTTCGCATCGCCAC | 59.66 | 60.15 | 193 | 111 |
100.00%
|
18.18%
|
| Primer 119 | Rothia | ATCGCGTGCTGTTCACCT | ACAGTACCTTCGCATCGCC | 59.66 | 60.15 | 195 | 111 |
100.00%
|
18.18%
|
| Primer 120 | Rothia | ATCGCGTGCTGTTCACCT | CAGTACCTTCGCATCGCCA | 59.66 | 60.15 | 194 | 111 |
100.00%
|
18.18%
|