| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 181 | Rothia | GCAGCCGACGAATGCAAA | TCTTTCTTGGTGGGTCGCC | 59.44 | 59.93 | 266 | 104 |
100.00%
|
18.18%
|
| Primer 182 | Rothia | GCAGCCGACGAATGCAAA | CGTTCACGTTTGGCGACTG | 59.44 | 60.08 | 239 | 104 |
100.00%
|
36.36%
|
| Primer 183 | Rothia | ATTGCACTGCCCTGCGTA | GCGATGAAGGGGACGTCTT | 59.65 | 59.78 | 284 | 103 |
100.00%
|
18.18%
|
| Primer 184 | Rothia | TGCTGCTCGAAATTGCGC | TGCGAATCAGCAGGTTGGT | 59.82 | 59.93 | 156 | 103 |
100.00%
|
18.18%
|
| Primer 185 | Rothia | CATGATCCGTTTGCACCGC | AGCGCAACAGGTCGAACT | 60.23 | 59.58 | 161 | 103 |
100.00%
|
27.27%
|
| Primer 186 | Rothia | TCACCGTGGCGTTGAAGA | ACCGTAACCGAACGCGAA | 59.18 | 59.67 | 188 | 103 |
100.00%
|
36.36%
|
| Primer 187 | Rothia | AATCGCTCACGGCATGCT | GAACCACAGCCTGGCTCTT | 60.12 | 59.93 | 290 | 103 |
100.00%
|
18.18%
|
| Primer 188 | Rothia | AATCGCTCACGGCATGCT | AACCACAGCCTGGCTCTT | 60.12 | 58.74 | 289 | 103 |
100.00%
|
18.18%
|
| Primer 189 | Rothia | AGGCTGCACGTATCGCAA | GTGCCTTCATCGGGAGCTT | 59.74 | 60.08 | 246 | 103 |
100.00%
|
45.45%
|
| Primer 190 | Rothia | TGCTTTACGGTCTGCGCT | CCAAACCCAGTTCACACGC | 59.66 | 59.64 | 152 | 103 |
100.00%
|
36.36%
|