| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 221 | Rothia | TGCAAAGTCGGTTCCGCT | ACCATCAACCCAGCTCGTG | 59.89 | 60.00 | 202 | 95 |
100.00%
|
18.18%
|
| Primer 222 | Rothia | TGCAAAGTCGGTTCCGCT | CGTCACCATCAACCCAGCT | 59.89 | 60.00 | 206 | 95 |
100.00%
|
18.18%
|
| Primer 223 | Rothia | TCGGCAAGGAATTCCGCA | TTCTTCGGCAGCAGCGAA | 59.65 | 59.97 | 295 | 95 |
100.00%
|
45.45%
|
| Primer 224 | Rothia | ATTGGTGACGCCTGCGAA | TAGACACCGCAACGTCCAC | 59.97 | 60.01 | 214 | 95 |
100.00%
|
18.18%
|
| Primer 225 | Rothia | ATTGGTGACGCCTGCGAA | GTAGACACCGCAACGTCCA | 59.97 | 60.01 | 215 | 95 |
100.00%
|
18.18%
|
| Primer 226 | Rothia | TGTTCGCTTGGCTGGGTA | AGGACGAATGCGCATGCT | 58.85 | 60.12 | 289 | 95 |
100.00%
|
27.27%
|
| Primer 227 | Rothia | ATCGACTTCGGCTTCGTGG | AGGACGAATGCGCATGCT | 60.15 | 60.12 | 188 | 95 |
100.00%
|
27.27%
|
| Primer 228 | Rothia | TTCAAGAACCGCACCGCT | AGCCGTAACGCAGAACCA | 59.89 | 59.26 | 298 | 95 |
100.00%
|
36.36%
|
| Primer 229 | Rothia | AGCAGGACGACGAGCAAAA | ATGCGCAGGTGAACTCGT | 59.93 | 59.66 | 192 | 94 |
100.00%
|
36.36%
|
| Primer 230 | Rothia | AAGCAGGACGACGAGCAAA | ATGCGCAGGTGAACTCGT | 59.93 | 59.66 | 193 | 94 |
100.00%
|
45.45%
|