| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 251 | Rothia | TCACAAGCCGAACGAGTCC | TTTCGACGCGAGGTGCAT | 60.01 | 60.05 | 257 | 93 |
100.00%
|
18.18%
|
| Primer 252 | Rothia | GCCGTACCCCTCGAAAACA | TCGATGCGCATGAGCTGT | 60.00 | 59.81 | 164 | 93 |
100.00%
|
18.18%
|
| Primer 253 | Rothia | AGACGCTCTGCAGGAACTC | TCAGCAACCGCATCGTGT | 59.41 | 59.97 | 219 | 92 |
100.00%
|
27.27%
|
| Primer 254 | Rothia | TTGCCGTCGTGCTTGTGT | CACCCAGACACGAGCATGT | 60.51 | 60.00 | 164 | 92 |
100.00%
|
18.18%
|
| Primer 255 | Rothia | GACTGTCGAGGGCAACGAT | ACTGGGACTCATTGAGCGC | 59.78 | 60.08 | 248 | 92 |
100.00%
|
18.18%
|
| Primer 256 | Rothia | AGCAGGCGAAACCGAAGA | ACCACGGAGGACTTCAGGT | 59.26 | 60.15 | 234 | 92 |
100.00%
|
36.36%
|
| Primer 257 | Rothia | TGCGATTGTTGCCACCCA | ATCCTCGGGTGCCAGAAGA | 60.20 | 60.31 | 217 | 92 |
100.00%
|
18.18%
|
| Primer 258 | Rothia | TGAGCTGAAGACCGCAACC | AACCAGATGCGCTAGACCG | 60.30 | 59.86 | 172 | 91 |
100.00%
|
18.18%
|
| Primer 259 | Rothia | TGGCGTTTCTGGCGTACA | TCCTTAATGCTGCGCACCA | 59.58 | 60.00 | 232 | 91 |
100.00%
|
18.18%
|
| Primer 260 | Rothia | TGCGATTGTTGCCACCCA | CAAAACCCGAGTACGCTGC | 60.20 | 59.50 | 194 | 91 |
100.00%
|
18.18%
|