| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 301 | Rothia | TCGCTTCTGGCTCCCATTG | ATGCGCTTGTCCATCGGT | 60.08 | 59.73 | 266 | 84 |
100.00%
|
18.18%
|
| Primer 302 | Rothia | AGACCCTGCAAGACCTGGA | TTTGCTCGGGTGCATGGT | 60.15 | 59.88 | 221 | 84 |
100.00%
|
18.18%
|
| Primer 303 | Rothia | TGTGCAGCTCGCCAAGAA | TGCCAAGCGCATGGAAAG | 59.89 | 59.04 | 248 | 84 |
100.00%
|
18.18%
|
| Primer 304 | Rothia | TCTCGGCATGGGTATTGGC | TGCACCGGTTTCAACGCT | 59.85 | 60.52 | 244 | 84 |
100.00%
|
27.27%
|
| Primer 305 | Rothia | CGCAACCCTGAAGAAGGCT | AGTGTGCGAAGGCCTTGT | 60.30 | 59.49 | 284 | 84 |
100.00%
|
45.45%
|
| Primer 306 | Rothia | CGCAACCCTGAAGAAGGCT | TGCGAAGGCCTTGTTGGA | 60.30 | 59.49 | 280 | 84 |
100.00%
|
45.45%
|
| Primer 307 | Rothia | TGGACAAGGATCCGGAGCT | ACGCTCGTCTTCACTGGTG | 60.31 | 60.01 | 176 | 84 |
100.00%
|
27.27%
|
| Primer 308 | Rothia | TCACCAACTTCGTGCCCA | ACCATCTTCGGGTAGGGCT | 59.09 | 60.00 | 187 | 84 |
100.00%
|
36.36%
|
| Primer 309 | Rothia | TGGTTTCGGCATGCTGGT | TCGTGAAGGCCTTGTCGTC | 59.88 | 60.01 | 266 | 84 |
100.00%
|
18.18%
|
| Primer 310 | Rothia | TGGTTTCGGCATGCTGGT | TGAAGGCCTTGTCGTCACC | 59.88 | 59.93 | 263 | 84 |
100.00%
|
18.18%
|