| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 331 | Rothia | GGATTGGCGTAGTCGTGCA | TCGTCTTCACTGGTGCCAC | 60.45 | 59.93 | 198 | 82 |
100.00%
|
27.27%
|
| Primer 332 | Rothia | CGCATCCTCACCAACGAGA | AACCAGATGCGCTAGACCG | 59.78 | 59.86 | 222 | 81 |
100.00%
|
18.18%
|
| Primer 333 | Rothia | ATCAGCGCTTGAAGGGTCC | ACGCAAAGCAGCAATCGC | 60.08 | 60.13 | 163 | 81 |
100.00%
|
18.18%
|
| Primer 334 | Rothia | TGCCGTTGATCGTGCACA | CTCGCGTTTGAGGTAGGCA | 60.28 | 60.08 | 167 | 81 |
100.00%
|
18.18%
|
| Primer 335 | Rothia | TGGCGTTTCTGGCGTACA | GCCCAATCCACGGGTAACA | 59.58 | 60.00 | 175 | 81 |
100.00%
|
18.18%
|
| Primer 336 | Rothia | TGGCGTTTCTGGCGTACA | TGCCCAATCCACGGGTAAC | 59.58 | 60.00 | 176 | 81 |
100.00%
|
18.18%
|
| Primer 337 | Rothia | CGCTGAAGACCCTGCAAGA | TTTGCTCGGGTGCATGGT | 60.00 | 59.88 | 227 | 81 |
100.00%
|
18.18%
|
| Primer 338 | Rothia | GTTGGCTCATGCGCATCTG | AGCGGATGCCAACAGCTT | 59.94 | 59.97 | 203 | 81 |
100.00%
|
18.18%
|
| Primer 339 | Rothia | TGCTTCGGGATCCTGATGC | AGCGGATGCCAACAGCTT | 59.85 | 59.97 | 230 | 81 |
100.00%
|
18.18%
|
| Primer 340 | Rothia | TCACCGCAACCGACGAAA | TGGACGAATTCCCAGGTGC | 59.89 | 60.00 | 244 | 81 |
100.00%
|
27.27%
|