| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 461 | Rothia | TGCCGTTGATCGTGCACA | TCTCGCGTTTGAGGTAGGC | 60.28 | 59.79 | 168 | 70 |
100.00%
|
18.18%
|
| Primer 462 | Rothia | AGAAGCTGCTGCCGAACA | ACGCCAGCCTTGTTAGCA | 59.57 | 59.57 | 187 | 70 |
100.00%
|
27.27%
|
| Primer 463 | Rothia | TGGCGTTGCTGCATGACA | AGTCGTTCAGGTGCAGCA | 60.59 | 59.18 | 291 | 70 |
100.00%
|
18.18%
|
| Primer 464 | Rothia | TGTGTGGACTGGTGAAGCC | AGCGATCTCGGCATGCTT | 59.85 | 59.50 | 220 | 69 |
100.00%
|
18.18%
|
| Primer 465 | Rothia | TTTCGGACGCGTACGCTT | ATCACCCTTGAGTGCCTGC | 60.05 | 60.00 | 230 | 69 |
100.00%
|
18.18%
|
| Primer 466 | Rothia | TTTCGGACGCGTACGCTT | GCACCTGATCCGTTGACCA | 60.05 | 60.00 | 192 | 69 |
100.00%
|
18.18%
|
| Primer 467 | Rothia | TTTCGGACGCGTACGCTT | ACAGACGACGAAGCACCTG | 60.05 | 60.01 | 204 | 69 |
100.00%
|
18.18%
|
| Primer 468 | Rothia | TGGGGTGTGGTGCAACTT | GCTGCAACGCGTTCAACA | 59.31 | 59.98 | 230 | 69 |
100.00%
|
18.18%
|
| Primer 469 | Rothia | TGGGGTGTGGTGCAACTT | TGCTGCAACGCGTTCAAC | 59.31 | 59.98 | 231 | 69 |
100.00%
|
18.18%
|
| Primer 470 | Rothia | ACGAAGAGACCGGTGTTGA | ACTGCGTCACCAACAGTCTT | 58.58 | 59.82 | 172 | 69 |
100.00%
|
54.55%
|