| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 61 | Rothia | AAGAATGCACGGCTGGCA | TGACGGAGGGGAGCTTCTT | 60.28 | 60.23 | 184 | 125 |
100.00%
|
18.18%
|
| Primer 62 | Rothia | TGGCCTCATCAAGCTGCA | AACGCCAGCAGCCTTCTT | 59.24 | 59.89 | 238 | 125 |
100.00%
|
36.36%
|
| Primer 63 | Rothia | AAGCTGCAGATCCAGGCA | AACGCCAGCAGCCTTCTT | 58.92 | 59.89 | 228 | 125 |
100.00%
|
45.45%
|
| Primer 64 | Rothia | ACGACGAATACGAGCTCGC | TCTTGCCGTGCATCAGCT | 60.30 | 59.65 | 228 | 125 |
100.00%
|
18.18%
|
| Primer 65 | Rothia | ACTCCGGTACTGTGCGTTG | ACCAGTGCCAATTGCCCA | 60.01 | 59.80 | 162 | 124 |
100.00%
|
18.18%
|
| Primer 66 | Rothia | TGAGCGTCGCACTTACCA | ACCAGTGCCAATTGCCCA | 58.96 | 59.80 | 238 | 124 |
100.00%
|
18.18%
|
| Primer 67 | Rothia | CTGAGCGTCGCACTTACCA | ACCAGTGCCAATTGCCCA | 60.08 | 59.80 | 239 | 124 |
100.00%
|
18.18%
|
| Primer 68 | Rothia | TTCGTGTTCACCCTCGTCG | TCTTGCCGTGCATCAGCT | 60.01 | 59.65 | 289 | 124 |
100.00%
|
18.18%
|
| Primer 69 | Rothia | ACTCGCATCGTCGCAACA | TCTGCAACAACGGCATCG | 60.05 | 58.75 | 151 | 124 |
100.00%
|
36.36%
|
| Primer 70 | Rothia | TGGCTCTGGGCATTGTGA | TCGTTGCCAAAGCCACCT | 58.83 | 59.81 | 168 | 122 |
100.00%
|
18.18%
|