| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 731 | Rothia | AACACCGCATCCCTGGAAG | CCTTGGGCTTGACGGGAAT | 60.00 | 60.00 | 172 | 50 |
100.00%
|
18.18%
|
| Primer 732 | Rothia | CGCGTTTGGGCATTGGAA | GCAATTCGCTACGCTCAGC | 59.35 | 60.01 | 189 | 50 |
100.00%
|
27.27%
|
| Primer 733 | Rothia | TCGAGGCGAAGATTGGCA | ACTCCATGTGCATGCGCT | 59.02 | 60.05 | 294 | 50 |
100.00%
|
36.36%
|
| Primer 734 | Rothia | AGCATACGCGTTCCTGCT | ACGAAGGTGTTCAGCGCA | 59.42 | 59.89 | 201 | 50 |
100.00%
|
27.27%
|
| Primer 735 | Rothia | TGGTCATTGTCGCCTCCAC | CGGTGACCAGGGTGTTGAA | 60.00 | 59.85 | 159 | 50 |
100.00%
|
18.18%
|
| Primer 736 | Rothia | TGCAGGCGTTGTGATGCT | ACTTGGTGTTGCGCAGGT | 60.28 | 60.12 | 251 | 50 |
100.00%
|
45.45%
|
| Primer 737 | Rothia | ACGCCTCGGCAAACTTCA | TGCGATGGCTTCCTTGGT | 59.89 | 59.24 | 224 | 50 |
100.00%
|
36.36%
|
| Primer 738 | Rothia | TGAACGCCTCGGCAAACT | TGCGATGGCTTCCTTGGT | 59.89 | 59.24 | 227 | 50 |
100.00%
|
36.36%
|
| Primer 739 | Rothia | ACCCGTACGCAAAGCTGT | AGCGGTCATTGCGGTGAA | 59.58 | 59.97 | 173 | 50 |
100.00%
|
27.27%
|
| Primer 740 | Rothia | TTCACCGCAATGACCGCT | TCCACTCACGCATACGCA | 59.97 | 59.03 | 244 | 50 |
100.00%
|
36.36%
|