| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Shewanella | ATGCCAAGACGTCGCGTT | TGTCCAGGGCTGCTTCAAG | 60.36 | 59.93 | 202 | 122 |
100.00%
|
58.82%
|
| Primer 2 | Shewanella | ATGCCAAGACGTCGCGTT | TGTCCAGGGCTGCTTCAA | 60.36 | 58.75 | 202 | 122 |
100.00%
|
58.82%
|
| Primer 3 | Shewanella | ATGCCAAGACGTCGCGTT | TTGTCCAGGGCTGCTTCA | 60.36 | 58.75 | 203 | 122 |
100.00%
|
58.82%
|
| Primer 4 | Shewanella | ATGCCAAGACGTCGCGTT | CCAGGGCTGCTTCAAGGAT | 60.36 | 59.69 | 199 | 122 |
100.00%
|
70.59%
|
| Primer 5 | Shewanella | ACGCCAAAGGATTCGGGT | TGACGCGTGCATCCCTTT | 59.25 | 59.97 | 155 | 120 |
100.00%
|
52.94%
|
| Primer 6 | Shewanella | ATGCCAAGACGTCGCGTT | GCGAACGTTATCCAGGGCT | 60.36 | 60.15 | 210 | 111 |
100.00%
|
47.06%
|
| Primer 7 | Shewanella | ATGCCAAGACGTCGCGTT | CCAGGGCTGCTTCAAGGAT | 60.36 | 59.69 | 199 | 111 |
100.00%
|
70.59%
|
| Primer 8 | Shewanella | ATGCCAAGACGTCGCGTT | ATCCAGGGCTGCTTCAAGG | 60.36 | 59.69 | 201 | 111 |
100.00%
|
64.71%
|
| Primer 9 | Shewanella | AGCAGTCATCGCCAAGCT | GACTTCAGCGTCGCGAGTA | 59.33 | 59.87 | 186 | 106 |
100.00%
|
58.82%
|
| Primer 10 | Shewanella | AGCAGTCATCGCCAAGCT | CCTGGAAACGTGGACCCAA | 59.33 | 59.85 | 226 | 106 |
100.00%
|
58.82%
|