| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Shewanella | CAACGCCAAAGGATTCGGG | TGACGCGTGCATCCCTTT | 59.49 | 59.97 | 157 | 82 |
100.00%
|
52.94%
|
| Primer 22 | Shewanella | CCCGTACGTCGCCTCAAAA | TGTGGCTGGTACGCTTGA | 60.37 | 58.86 | 279 | 82 |
100.00%
|
47.06%
|
| Primer 23 | Shewanella | AGGCATGCAAGCTGGTGA | ACGTAGAGTGGCGTATGCG | 59.56 | 59.94 | 202 | 80 |
100.00%
|
47.06%
|
| Primer 24 | Shewanella | GCCGTAGCAGATGAAGCCA | ATAACCTCGCCACCCATGC | 60.15 | 60.15 | 230 | 79 |
100.00%
|
52.94%
|
| Primer 25 | Shewanella | CCGTACTTTGCAGGGTCGT | GGACGACATTCGCGAATCG | 60.01 | 59.45 | 183 | 79 |
100.00%
|
58.82%
|
| Primer 26 | Shewanella | GGCAGCTAAAATCCGTCGTG | ACGCGATCAGCTTTGCCT | 59.63 | 60.05 | 246 | 78 |
100.00%
|
47.06%
|
| Primer 27 | Shewanella | ATGCCAAGACGTCGCGTT | CAAGGGCTGCCTCAAGGAT | 60.36 | 59.69 | 199 | 78 |
100.00%
|
58.82%
|
| Primer 28 | Shewanella | GTAACAACCGCTCACACGC | GGCACGAAGTTCAGCAACA | 59.80 | 58.98 | 176 | 75 |
91.67%
|
64.71%
|
| Primer 29 | Shewanella | GTAACAACCGCTCACACGC | GGCACGAAGTTCAGCAACAA | 59.80 | 59.62 | 176 | 75 |
91.67%
|
64.71%
|
| Primer 30 | Shewanella | CGGTGGTATCACTGGCCAA | TGTGGCTTACGACGTGCT | 59.70 | 59.27 | 171 | 72 |
100.00%
|
64.71%
|